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Optimization of a Protocol for Protein Extraction from Calcified Aortic Valves for Proteomics Applications: Development of a Standard Operating Procedure.
Trindade F, Ferreira AF, Saraiva F, Martins D, Mendes VM, Sousa C, Gavina C, Leite-Moreira A, Manadas B, Falcão-Pires I, Vitorino R. Trindade F, et al. Proteomes. 2022 Sep 1;10(3):30. doi: 10.3390/proteomes10030030. Proteomes. 2022. PMID: 36136308 Free PMC article.
Once we confirmed the superiority of the latter, we further optimized it by testing the effect of beads size, the number of homogenization cycles, tube capacity, lysis buffer/tissue mass ratio, and two different lysis buffers. Optimal protein extraction was achieved with 2 …
Once we confirmed the superiority of the latter, we further optimized it by testing the effect of beads size, the number of homogenization c …
The Enhanced Efficacy of Intracellular Delivery of Doxorubicin/C6-Ceramide Combination Mediated by the F3 Peptide/Nucleolin System Is Supported by the Downregulation of the PI3K/Akt Pathway.
Cruz AF, Caleiras MB, Fonseca NA, Gonçalves N, Mendes VM, Sampaio SF, Moura V, Melo JB, Almeida RD, Manadas B, Simões S, Moreira JN. Cruz AF, et al. Cancers (Basel). 2021 Jun 18;13(12):3052. doi: 10.3390/cancers13123052. Cancers (Basel). 2021. PMID: 34207464 Free PMC article.