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The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.
Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S. Götzke H, et al. Among authors: opazo f. Nat Commun. 2019 Sep 27;10(1):4403. doi: 10.1038/s41467-019-12301-7. Nat Commun. 2019. PMID: 31562305 Free PMC article.
Circumvention of common labelling artefacts using secondary nanobodies.
Sograte-Idrissi S, Schlichthaerle T, Duque-Afonso CJ, Alevra M, Strauss S, Moser T, Jungmann R, Rizzoli SO, Opazo F. Sograte-Idrissi S, et al. Among authors: opazo f. Nanoscale. 2020 May 14;12(18):10226-10239. doi: 10.1039/d0nr00227e. Nanoscale. 2020. PMID: 32356544
A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol.
Gerdes C, Waal N, Offner T, Fornasiero EF, Wender N, Verbarg H, Manzini I, Trenkwalder C, Mollenhauer B, Strohäker T, Zweckstetter M, Becker S, Rizzoli SO, Basmanav FB, Opazo F. Gerdes C, et al. Among authors: opazo f. Nat Commun. 2020 Jun 1;11(1):2729. doi: 10.1038/s41467-020-16575-0. Nat Commun. 2020. PMID: 32483166 Free PMC article.
Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells.
Oleksiievets N, Sargsyan Y, Thiele JC, Mougios N, Sograte-Idrissi S, Nevskyi O, Gregor I, Opazo F, Thoms S, Enderlein J, Tsukanov R. Oleksiievets N, et al. Among authors: opazo f. Commun Biol. 2022 Jan 11;5(1):38. doi: 10.1038/s42003-021-02976-4. Commun Biol. 2022. PMID: 35017652 Free PMC article.
A Versatile Synaptotagmin-1 Nanobody Provides Perturbation-Free Live Synaptic Imaging And Low Linkage-Error in Super-Resolution Microscopy.
Queiroz Zetune Villa Real K, Mougios N, Rehm R, Sograte-Idrissi S, Albert L, Rahimi AM, Maidorn M, Hentze J, Martínez-Carranza M, Hosseini H, Saal KA, Oleksiievets N, Prigge M, Tsukanov R, Stenmark P, Fornasiero EF, Opazo F. Queiroz Zetune Villa Real K, et al. Among authors: opazo f. Small Methods. 2023 Oct;7(10):e2300218. doi: 10.1002/smtd.202300218. Epub 2023 Jul 8. Small Methods. 2023. PMID: 37421204
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, Opazo F, Fornasiero EF, Jungmann R. Unterauer EM, et al. Among authors: opazo f. Cell. 2024 Mar 28;187(7):1785-1800.e16. doi: 10.1016/j.cell.2024.02.045. Cell. 2024. PMID: 38552614
60 results