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Effect of size, quaternary structure and translational error on the static and dynamic heterogeneity of beta-galactosidase and measurement of electrophoretic dynamic heterogeneity.
Protein J. 2010 Aug;29(6):398-406. doi: 10.1007/s10930-010-9266-0.
Protein J. 2010.
PMID: 20607374
Single molecule assays of beta-galactosidase from two wild-type strains of E. coli: effects of protease inhibitors on microheterogeneity and different relative activities with differing substrates.
Nichols ER, Gavina JM, McLeod RG, Craig DB.
Nichols ER, et al.
Protein J. 2007 Feb;26(2):95-105. doi: 10.1007/s10930-006-9050-3.
Protein J. 2007.
PMID: 17253128
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Single molecule assays reveal differences between in vitro and in vivo synthesized beta-galactosidase.
Nichols ER, Craig DB.
Nichols ER, et al.
Protein J. 2008 Sep;27(6):376-83. doi: 10.1007/s10930-008-9147-y.
Protein J. 2008.
PMID: 18769886
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Measurement of the differences in electrophoretic mobilities of individual molecules of E. coli beta-galactosidase provides insight into structural differences which underlie enzyme microheterogeneity.
Nichols ER, Craig DB.
Nichols ER, et al.
Electrophoresis. 2008 Nov;29(20):4257-69. doi: 10.1002/elps.200800060.
Electrophoresis. 2008.
PMID: 18924101
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Continuous flow assay for the simultaneous measurement of the electrophoretic mobility, catalytic activity and its variation over time of individual molecules of Escherichia coli beta-galactosidase.
Craig DB, Nichols ER.
Craig DB, et al. Among authors: nichols er.
Electrophoresis. 2008 Nov;29(21):4298-303. doi: 10.1002/elps.200800482.
Electrophoresis. 2008.
PMID: 19016504
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Effect of alteration of translation error rate on enzyme microheterogeneity as assessed by variation in single molecule electrophoretic mobility and catalytic activity.
Nichols ER, Shadabi E, Craig DB.
Nichols ER, et al.
Biochem Cell Biol. 2009 Jun;87(3):517-29. doi: 10.1139/o09-010.
Biochem Cell Biol. 2009.
PMID: 19448745
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Kinetic studies of unmodified individual Escherichia coli beta-galactosidase molecules in free solution.
Craig DB, Haslam AM, Coombs JM, Nichols ER.
Craig DB, et al. Among authors: nichols er.
Biochem Cell Biol. 2010 Jun;88(3):451-8. doi: 10.1139/o09-120.
Biochem Cell Biol. 2010.
PMID: 20555387
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