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A convenient protocol for establishing a human cell culture model of the outer retina.
Lynn SA, Keeling E, Dewing JM, Johnston DA, Page A, Cree AJ, Tumbarello DA, Newman TA, Lotery AJ, Ratnayaka JA. Lynn SA, et al. F1000Res. 2018 Jul 18;7:1107. doi: 10.12688/f1000research.15409.1. eCollection 2018. F1000Res. 2018. PMID: 30271583 Free PMC article.
This in-vitro model therefore has the benefit of achieving the 3Rs objective of reducing and replacing the use of animals in research. As well as recapitulating salient structural and physiological features of native RPE, other advantages of this model include its simplici …
This in-vitro model therefore has the benefit of achieving the 3Rs objective of reducing and replacing the use of animals in research …
Ex-vivo models of the Retinal Pigment Epithelium (RPE) in long-term culture faithfully recapitulate key structural and physiological features of native RPE.
Lynn SA, Ward G, Keeling E, Scott JA, Cree AJ, Johnston DA, Page A, Cuan-Urquizo E, Bhaskar A, Grossel MC, Tumbarello DA, Newman TA, Lotery AJ, Ratnayaka JA. Lynn SA, et al. Tissue Cell. 2017 Aug;49(4):447-460. doi: 10.1016/j.tice.2017.06.003. Epub 2017 Jun 19. Tissue Cell. 2017. PMID: 28669519 Free PMC article.