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A bacterial genetic selection system for ubiquitylation cascade discovery.
Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T, Katina C, Pilzer I, Ben-Aroya S, Prag G. Levin-Kravets O, et al. Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 2016 Oct 3. Nat Methods. 2016. PMID: 27694912
Synthetic biology approach to reconstituting the ubiquitylation cascade in bacteria.
Keren-Kaplan T, Attali I, Motamedchaboki K, Davis BA, Tanner N, Reshef Y, Laudon E, Kolot M, Levin-Kravets O, Kleifeld O, Glickman M, Horazdovsky BF, Wolf DA, Prag G. Keren-Kaplan T, et al. Among authors: levin kravets o. EMBO J. 2012 Jan 18;31(2):378-90. doi: 10.1038/emboj.2011.397. Epub 2011 Nov 11. EMBO J. 2012. PMID: 22081111 Free PMC article.
Structure of ubiquitylated-Rpn10 provides insight into its autoregulation mechanism.
Keren-Kaplan T, Zeev Peters L, Levin-Kravets O, Attali I, Kleifeld O, Shohat N, Artzi S, Zucker O, Pilzer I, Reis N, Glickman MH, Ben-Aroya S, Prag G. Keren-Kaplan T, et al. Among authors: levin kravets o. Nat Commun. 2016 Oct 4;7:12960. doi: 10.1038/ncomms12960. Nat Commun. 2016. PMID: 27698474 Free PMC article.
Ubiquitylation-dependent oligomerization regulates activity of Nedd4 ligases.
Attali I, Tobelaim WS, Persaud A, Motamedchaboki K, Simpson-Lavy KJ, Mashahreh B, Levin-Kravets O, Keren-Kaplan T, Pilzer I, Kupiec M, Wiener R, Wolf DA, Rotin D, Prag G. Attali I, et al. Among authors: levin kravets o. EMBO J. 2017 Feb 15;36(4):425-440. doi: 10.15252/embj.201694314. Epub 2017 Jan 9. EMBO J. 2017. PMID: 28069708 Free PMC article.
Split Chloramphenicol Acetyl-Transferase Assay Reveals Self-Ubiquitylation-Dependent Regulation of UBE3B.
Levin-Kravets O, Kordonsky A, Shusterman A, Biswas S, Persaud A, Elias S, Langut Y, Florentin A, Simpson-Lavy KJ, Yariv E, Avishid R, Sror M, Almog O, Marshanski T, Kadosh S, Ben David N, Manori B, Fischer Z, Lilly J, Borisova E, Ambrozkiewicz MC, Tarabykin V, Kupiec M, Thaker M, Rotin D, Prag G. Levin-Kravets O, et al. J Mol Biol. 2021 Nov 19;433(23):167276. doi: 10.1016/j.jmb.2021.167276. Epub 2021 Sep 29. J Mol Biol. 2021. PMID: 34599943 Free article.