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Improved two-stage protein expression and purification via autoinduction of both autolysis and auto DNA/RNA hydrolysis conferred by phage lysozyme and DNA/RNA endonuclease.
Biotechnol Bioeng. 2020 Sep;117(9):2852-2860. doi: 10.1002/bit.27444. Epub 2020 Jun 20.
Biotechnol Bioeng. 2020.
PMID: 32462658
Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion.
Menacho-Melgar R, Ye Z, Moreb EA, Yang T, Efromson JP, Decker JS, Wang R, Lynch MD.
Menacho-Melgar R, et al.
Biotechnol Bioeng. 2020 Sep;117(9):2715-2727. doi: 10.1002/bit.27440. Epub 2020 Jun 10.
Biotechnol Bioeng. 2020.
PMID: 32441815
Free PMC article.
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