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Characterization and engineering of a two-enzyme system for plastics depolymerization.
Proc Natl Acad Sci U S A. 2020 Oct 13;117(41):25476-25485. doi: 10.1073/pnas.2006753117. Epub 2020 Sep 28.
Proc Natl Acad Sci U S A. 2020.
PMID: 32989159
Free PMC article.
Enabling microbial syringol conversion through structure-guided protein engineering.
Machovina MM, Mallinson SJB, Knott BC, Meyers AW, Garcia-Borràs M, Bu L, Gado JE, Oliver A, Schmidt GP, Hinchen DJ, Crowley MF, Johnson CW, Neidle EL, Payne CM, Houk KN, Beckham GT, McGeehan JE, DuBois JL.
Machovina MM, et al. Among authors: gado je.
Proc Natl Acad Sci U S A. 2019 Jul 9;116(28):13970-13976. doi: 10.1073/pnas.1820001116. Epub 2019 Jun 24.
Proc Natl Acad Sci U S A. 2019.
PMID: 31235604
Free PMC article.
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Improving Enzyme Optimum Temperature Prediction with Resampling Strategies and Ensemble Learning.
Gado JE, Beckham GT, Payne CM.
Gado JE, et al.
J Chem Inf Model. 2020 Aug 24;60(8):4098-4107. doi: 10.1021/acs.jcim.0c00489. Epub 2020 Jul 22.
J Chem Inf Model. 2020.
PMID: 32639729
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Machine learning reveals sequence-function relationships in family 7 glycoside hydrolases.
Gado JE, Harrison BE, Sandgren M, Ståhlberg J, Beckham GT, Payne CM.
Gado JE, et al.
J Biol Chem. 2021 Aug;297(2):100931. doi: 10.1016/j.jbc.2021.100931. Epub 2021 Jul 1.
J Biol Chem. 2021.
PMID: 34216620
Free PMC article.
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Enzyme kinetics by GH7 cellobiohydrolases on chromogenic substrates is dictated by non-productive binding: insights from crystal structures and MD simulation.
Haataja T, Gado JE, Nutt A, Anderson NT, Nilsson M, Momeni MH, Isaksson R, Väljamäe P, Johansson G, Payne CM, Ståhlberg J.
Haataja T, et al. Among authors: gado je.
FEBS J. 2023 Jan;290(2):379-399. doi: 10.1111/febs.16602. Epub 2022 Sep 6.
FEBS J. 2023.
PMID: 35997626
Free PMC article.
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Sourcing thermotolerant poly(ethylene terephthalate) hydrolase scaffolds from natural diversity.
Erickson E, Gado JE, Avilán L, Bratti F, Brizendine RK, Cox PA, Gill R, Graham R, Kim DJ, König G, Michener WE, Poudel S, Ramirez KJ, Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, Beckham GT, McGeehan JE.
Erickson E, et al. Among authors: gado je.
Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
Nat Commun. 2022.
PMID: 36543766
Free PMC article.
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Natural diversity screening, assay development, and characterization of nylon-6 enzymatic depolymerization.
Bell EL, Rosetto G, Ingraham MA, Ramirez KJ, Lincoln C, Clarke RW, Gado JE, Lilly JL, Kucharzyk KH, Erickson E, Beckham GT.
Bell EL, et al. Among authors: gado je.
Nat Commun. 2024 Feb 9;15(1):1217. doi: 10.1038/s41467-024-45523-5.
Nat Commun. 2024.
PMID: 38336849
Free PMC article.
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