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Histidine 407, a phantom residue in the E1 subunit of the Escherichia coli pyruvate dehydrogenase complex, activates reductive acetylation of lipoamide on the E2 subunit. An explanation for conservation of active sites between the E1 subunit and transketolase.
Nemeria N, Arjunan P, Brunskill A, Sheibani F, Wei W, Yan Y, Zhang S, Jordan F, Furey W. Nemeria N, et al. Among authors: furey w. Biochemistry. 2002 Dec 31;41(52):15459-67. doi: 10.1021/bi0205909. Biochemistry. 2002. PMID: 12501174
Glutamate 636 of the Escherichia coli pyruvate dehydrogenase-E1 participates in active center communication and behaves as an engineered acetolactate synthase with unusual stereoselectivity.
Nemeria N, Tittmann K, Joseph E, Zhou L, Vazquez-Coll MB, Arjunan P, Hübner G, Furey W, Jordan F. Nemeria N, et al. Among authors: furey w. J Biol Chem. 2005 Jun 3;280(22):21473-82. doi: 10.1074/jbc.M502691200. Epub 2005 Mar 31. J Biol Chem. 2005. PMID: 15802265 Free article.
A thiamin-bound, pre-decarboxylation reaction intermediate analogue in the pyruvate dehydrogenase E1 subunit induces large scale disorder-to-order transformations in the enzyme and reveals novel structural features in the covalently bound adduct.
Arjunan P, Sax M, Brunskill A, Chandrasekhar K, Nemeria N, Zhang S, Jordan F, Furey W. Arjunan P, et al. Among authors: furey w. J Biol Chem. 2006 Jun 2;281(22):15296-303. doi: 10.1074/jbc.M600656200. Epub 2006 Mar 10. J Biol Chem. 2006. PMID: 16531404 Free article.
Communication between thiamin cofactors in the Escherichia coli pyruvate dehydrogenase complex E1 component active centers: evidence for a "direct pathway" between the 4'-aminopyrimidine N1' atoms.
Nemeria NS, Arjunan P, Chandrasekhar K, Mossad M, Tittmann K, Furey W, Jordan F. Nemeria NS, et al. Among authors: furey w. J Biol Chem. 2010 Apr 9;285(15):11197-209. doi: 10.1074/jbc.M109.069179. Epub 2010 Jan 27. J Biol Chem. 2010. PMID: 20106967 Free PMC article.
102 results