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Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion.
Biotechnol Bioeng. 2020 Sep;117(9):2715-2727. doi: 10.1002/bit.27440. Epub 2020 Jun 10.
Biotechnol Bioeng. 2020.
PMID: 32441815
Free PMC article.
Media Robustness and Scalability of Phosphate Regulated Promoters Useful for Two-Stage Autoinduction in E. coli.
Moreb EA, Ye Z, Efromson JP, Hennigan JN, Menacho-Melgar R, Lynch MD.
Moreb EA, et al. Among authors: efromson jp.
ACS Synth Biol. 2020 Jun 19;9(6):1483-1486. doi: 10.1021/acssynbio.0c00182. Epub 2020 May 27.
ACS Synth Biol. 2020.
PMID: 32353228
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Improved two-stage protein expression and purification via autoinduction of both autolysis and auto DNA/RNA hydrolysis conferred by phage lysozyme and DNA/RNA endonuclease.
Menacho-Melgar R, Moreb EA, Efromson JP, Yang T, Hennigan JN, Wang R, Lynch MD.
Menacho-Melgar R, et al. Among authors: efromson jp.
Biotechnol Bioeng. 2020 Sep;117(9):2852-2860. doi: 10.1002/bit.27444. Epub 2020 Jun 20.
Biotechnol Bioeng. 2020.
PMID: 32462658
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A Review of the Biotechnological Production of Methacrylic Acid.
Lebeau J, Efromson JP, Lynch MD.
Lebeau J, et al. Among authors: efromson jp.
Front Bioeng Biotechnol. 2020 Mar 20;8:207. doi: 10.3389/fbioe.2020.00207. eCollection 2020.
Front Bioeng Biotechnol. 2020.
PMID: 32266236
Free PMC article.
Review.
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BioSamplr: An open source, low cost automated sampling system for bioreactors.
Efromson JP, Li S, Lynch MD.
Efromson JP, et al.
HardwareX. 2021 Feb 5;9:e00177. doi: 10.1016/j.ohx.2021.e00177. eCollection 2021 Apr.
HardwareX. 2021.
PMID: 35492036
Free PMC article.
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