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SMA-PAGE: A new method to examine complexes of membrane proteins using SMALP nano-encapsulation and native gel electrophoresis.
Pollock NL, Rai M, Simon KS, Hesketh SJ, Teo ACK, Parmar M, Sridhar P, Collins R, Lee SC, Stroud ZN, Bakker SE, Muench SP, Barton CH, Hurlbut G, Roper DI, Smith CJI, Knowles TJ, Spickett CM, East JM, Postis VLG, Dafforn TR. Pollock NL, et al. Among authors: dafforn tr. Biochim Biophys Acta Biomembr. 2019 Aug 1;1861(8):1437-1445. doi: 10.1016/j.bbamem.2019.05.011. Epub 2019 May 28. Biochim Biophys Acta Biomembr. 2019. PMID: 31150633 Free article.
Membrane protein extraction and purification using partially-esterified SMA polymers.
Hawkins OP, Jahromi CPT, Gulamhussein AA, Nestorow S, Bahra T, Shelton C, Owusu-Mensah QK, Mohiddin N, O'Rourke H, Ajmal M, Byrnes K, Khan M, Nahar NN, Lim A, Harris C, Healy H, Hasan SW, Ahmed A, Evans L, Vaitsopoulou A, Akram A, Williams C, Binding J, Thandi RK, Joby A, Guest A, Tariq MZ, Rasool F, Cavanagh L, Kang S, Asparuhov B, Jestin A, Dafforn TR, Simms J, Bill RM, Goddard AD, Rothnie AJ. Hawkins OP, et al. Among authors: dafforn tr. Biochim Biophys Acta Biomembr. 2021 Dec 1;1863(12):183758. doi: 10.1016/j.bbamem.2021.183758. Epub 2021 Sep 1. Biochim Biophys Acta Biomembr. 2021. PMID: 34480878 Free PMC article.
Ligand-induced conformational changes in a SMALP-encapsulated GPCR.
Routledge SJ, Jamshad M, Little HA, Lin YP, Simms J, Thakker A, Spickett CM, Bill RM, Dafforn TR, Poyner DR, Wheatley M. Routledge SJ, et al. Among authors: dafforn tr. Biochim Biophys Acta Biomembr. 2020 Jun 1;1862(6):183235. doi: 10.1016/j.bbamem.2020.183235. Epub 2020 Feb 29. Biochim Biophys Acta Biomembr. 2020. PMID: 32126232 Free PMC article.
Encapsulated membrane proteins: A simplified system for molecular simulation.
Lee SC, Khalid S, Pollock NL, Knowles TJ, Edler K, Rothnie AJ, R T Thomas O, Dafforn TR. Lee SC, et al. Among authors: dafforn tr. Biochim Biophys Acta. 2016 Oct;1858(10):2549-2557. doi: 10.1016/j.bbamem.2016.02.039. Epub 2016 Mar 3. Biochim Biophys Acta. 2016. PMID: 26946242 Free article. Review.
Differences in SMA-like polymer architecture dictate the conformational changes exhibited by the membrane protein rhodopsin encapsulated in lipid nano-particles.
Grime RL , Logan RT , Nestorow SA , Sridhar P , Edwards PC , Tate CG , Klumperman B , Dafforn TR , Poyner DR , Reeves PJ , Wheatley M . Grime RL , et al. Among authors: dafforn tr. Nanoscale. 2021 Aug 21;13(31):13519-13528. doi: 10.1039/d1nr02419a. Epub 2021 Aug 2. Nanoscale. 2021. PMID: 34477756 Free PMC article.
141 results