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Protein engineering of a ubiquitin-variant inhibitor of APC/C identifies a cryptic K48 ubiquitin chain binding site.
Watson ER, Grace CRR, Zhang W, Miller DJ, Davidson IF, Prabu JR, Yu S, Bolhuis DL, Kulko ET, Vollrath R, Haselbach D, Stark H, Peters JM, Brown NG, Sidhu SS, Schulman BA. Watson ER, et al. Among authors: brown ng. Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17280-17289. doi: 10.1073/pnas.1902889116. Epub 2019 Jul 26. Proc Natl Acad Sci U S A. 2019. PMID: 31350353 Free PMC article.
Ubiquitin chain-elongating enzyme UBE2S activates the RING E3 ligase APC/C for substrate priming.
Martinez-Chacin RC, Bodrug T, Bolhuis DL, Kedziora KM, Bonacci T, Ordureau A, Gibbs ME, Weissmann F, Qiao R, Grant GD, Cook JG, Peters JM, Wade Harper J, Emanuele MJ, Brown NG. Martinez-Chacin RC, et al. Among authors: brown ng. Nat Struct Mol Biol. 2020 Jun;27(6):550-560. doi: 10.1038/s41594-020-0424-6. Epub 2020 May 11. Nat Struct Mol Biol. 2020. PMID: 32393902 Free PMC article.
In silico APC/C substrate discovery reveals cell cycle-dependent degradation of UHRF1 and other chromatin regulators.
Franks JL, Martinez-Chacin RC, Wang X, Tiedemann RL, Bonacci T, Choudhury R, Bolhuis DL, Enrico TP, Mouery RD, Damrauer JS, Yan F, Harrison JS, Major MB, Hoadley KA, Suzuki A, Rothbart SB, Brown NG, Emanuele MJ. Franks JL, et al. Among authors: brown ng. PLoS Biol. 2020 Dec 11;18(12):e3000975. doi: 10.1371/journal.pbio.3000975. eCollection 2020 Dec. PLoS Biol. 2020. PMID: 33306668 Free PMC article.
Cryo-EM structure of the plant 26S proteasome.
Kandolf S, Grishkovskaya I, Belačić K, Bolhuis DL, Amann S, Foster B, Imre R, Mechtler K, Schleiffer A, Tagare HD, Zhong ED, Meinhart A, Brown NG, Haselbach D. Kandolf S, et al. Among authors: brown ng. Plant Commun. 2022 May 9;3(3):100310. doi: 10.1016/j.xplc.2022.100310. Epub 2022 Mar 11. Plant Commun. 2022. PMID: 35576154 Free PMC article.
83 results