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Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes.
Engmann AK, Hatch JJ, Nanda P, Veeraraghavan P, Ozkan A, Poulopoulos A, Murphy AJ, Macklis JD. Engmann AK, et al. Nat Protoc. 2022 Feb;17(2):222-251. doi: 10.1038/s41596-021-00638-7. Epub 2022 Jan 12. Nat Protoc. 2022. PMID: 35022617 Free PMC article. Review.
An evolutionarily acquired microRNA shapes development of mammalian cortical projections.
Diaz JL, Siththanandan VB, Lu V, Gonzalez-Nava N, Pasquina L, MacDonald JL, Woodworth MB, Ozkan A, Nair R, He Z, Sahni V, Sarnow P, Palmer TD, Macklis JD, Tharin S. Diaz JL, et al. Proc Natl Acad Sci U S A. 2020 Nov 17;117(46):29113-29122. doi: 10.1073/pnas.2006700117. Epub 2020 Nov 2. Proc Natl Acad Sci U S A. 2020. PMID: 33139574 Free PMC article.