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Ligand-induced conformational changes in a SMALP-encapsulated GPCR.
Biochim Biophys Acta Biomembr. 2020 Jun 1;1862(6):183235. doi: 10.1016/j.bbamem.2020.183235. Epub 2020 Feb 29.
Biochim Biophys Acta Biomembr. 2020.
PMID: 32126232
Free PMC article.
The synthesis of recombinant membrane proteins in yeast for structural studies.
Routledge SJ, Mikaliunaite L, Patel A, Clare M, Cartwright SP, Bawa Z, Wilks MD, Low F, Hardy D, Rothnie AJ, Bill RM.
Routledge SJ, et al.
Methods. 2016 Feb 15;95:26-37. doi: 10.1016/j.ymeth.2015.09.027. Epub 2015 Sep 30.
Methods. 2016.
PMID: 26431670
Free article.
Review.
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Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks.
Bawa Z, Routledge SJ, Jamshad M, Clare M, Sarkar D, Dickerson I, Ganzlin M, Poyner DR, Bill RM.
Bawa Z, et al.
Microb Cell Fact. 2014 Sep 4;13(1):127. doi: 10.1186/s12934-014-0127-y.
Microb Cell Fact. 2014.
PMID: 25186468
Free PMC article.
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