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Improving the Activity of Tryptophan Synthetase via a Nucleic Acid Scaffold.
Wang Y, Wang X, Niu S, Cheng W, Liu X, Min Y, Qiu Y, Ma L, Rao B, Zhu L. Wang Y, et al. Molecules. 2023 Oct 26;28(21):7272. doi: 10.3390/molecules28217272. Molecules. 2023. PMID: 37959692 Free PMC article.
The recombinant TSase constructed in this study with dCE fusion had the advantages of higher heat resistance and higher activity, and confirmed the feasibility of adding a nucleic acid scaffold, providing a new idea for the improvement of structurally similar enzymes....
The recombinant TSase constructed in this study with dCE fusion had the advantages of higher heat resistance and higher activity, and confir …
A Fusion of Taq DNA Polymerase with the CL7 Protein from Escherichia coli Remarkably Improves DNA Amplification.
Li Z, Wang Y, Wang X, Niu S, Su Z, Wang F, Ni J, Gong Y, Rao B. Li Z, et al. Molecules. 2024 Mar 4;29(5):1145. doi: 10.3390/molecules29051145. Molecules. 2024. PMID: 38474657 Free PMC article.
Modern diagnostics, molecular biology, and genetic engineering require DNA polymerases with improved performance. This study aimed to obtain and characterize a new CL7-Taq fusion DNA polymerase, in which the DNA coding sequence of Taq DNA polymerase was fused with that of …
Modern diagnostics, molecular biology, and genetic engineering require DNA polymerases with improved performance. This study aimed to obtain …