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Rational Design of Daunorubicin C-14 Hydroxylase Based on the Understanding of Its Substrate-Binding Mechanism.
Zhang J, Gao LX, Chen W, Zhong JJ, Qian C, Zhou WW. Zhang J, et al. Int J Mol Sci. 2023 May 6;24(9):8337. doi: 10.3390/ijms24098337. Int J Mol Sci. 2023. PMID: 37176043 Free PMC article.
Herein, the DoxA from Streptomyces peucetius subsp. caesius ATCC 27952 was expressed in Escherichia coli, and the rational design strategy was further applied to improve the enzyme activity. Eight amino acid residues were identified as the key sites via molecular docking. …
Herein, the DoxA from Streptomyces peucetius subsp. caesius ATCC 27952 was expressed in Escherichia coli, and the rational design strategy w …
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