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Exploring the Molecular Basis of Substrate and Product Selectivities of Nocardicin Bifunctional Thioesterase.
Yu Q, Xie L, Li Y, Bai L, Zhao YL, Wei D, Shi T. Yu Q, et al. Interdiscip Sci. 2022 Mar;14(1):233-244. doi: 10.1007/s12539-021-00482-z. Epub 2021 Oct 26. Interdiscip Sci. 2022. PMID: 34699036
The studies on binding affinities and loading conformations elucidated the important roles of peptide length and beta-lactam ring in substrate selectivity. Through energy decomposition and interaction analyses, some key residues involved in substrate selectivity were captu …
The studies on binding affinities and loading conformations elucidated the important roles of peptide length and beta-lactam ring in substra …
Mineralization of the herbicide swep by a two-strain consortium and characterization of a new amidase for hydrolyzing swep.
Zhang L, Hang P, Zhou X, Dai C, He Z, Jiang J. Zhang L, et al. Microb Cell Fact. 2020 Jan 7;19(1):4. doi: 10.1186/s12934-020-1276-9. Microb Cell Fact. 2020. PMID: 31910844 Free PMC article.
Gly449 and Val266 were confirmed by sequential error prone PCR to be the key catalytic sites for Ppa in the conversion of swep. CONCLUSIONS: These results provide additional microbial resources for the potential remediation of swep-contaminated sites and add new insights i …
Gly449 and Val266 were confirmed by sequential error prone PCR to be the key catalytic sites for Ppa in the conversion of swep. CONCL …
Understand the Specific Regio- and Enantioselectivity of Fluostatin Conjugation in the Post-Biosynthesis.
Wang Y, Zhang C, Zhao YL, Zhao R, Houk KN. Wang Y, et al. Biomolecules. 2020 May 26;10(6):815. doi: 10.3390/biom10060815. Biomolecules. 2020. PMID: 32466453 Free PMC article.
In this work, the unusual regio- and enantioselectivities were rationalized by density functional theory calculations with the M06-2X (SMD, water)/6-311 + G(d,p)//6-31G(d) method. These DFT calculations reproduce the lowest energy C1-(R)-C10'-(S) coupling pathway observed …
In this work, the unusual regio- and enantioselectivities were rationalized by density functional theory calculations with the M06-2X (SMD, …
Preferential catabolism of the (S)-enantiomer of the herbicide napropamide mediated by the enantioselective amidohydrolase SnaH and the dioxygenase Snpd in Sphingobium sp. strain B2.
Huang J, Chen D, Jiang J. Huang J, et al. Environ Microbiol. 2020 Jan;22(1):286-296. doi: 10.1111/1462-2920.14847. Epub 2019 Nov 14. Environ Microbiol. 2020. PMID: 31667998
The (R)- and (S)-enantiomers of the chiral herbicide napropamide (NAP) show different biological activities and ecotoxicities. ...B2, which was capable of catabolizing both NAP enantiomers. Compared with (R)-NAP, (S)-NAP was much more rapidly transformed by the amid …
The (R)- and (S)-enantiomers of the chiral herbicide napropamide (NAP) show different biological activities and ecotoxicities. ...B2, …
A Nag-like dioxygenase initiates 3,4-dichloronitrobenzene degradation via 4,5-dichlorocatechol in Diaphorobacter sp. strain JS3050.
Gao YZ, Palatucci ML, Waidner LA, Li T, Guo Y, Spain JC, Zhou NY. Gao YZ, et al. Environ Microbiol. 2021 Feb;23(2):1053-1065. doi: 10.1111/1462-2920.15295. Epub 2020 Nov 10. Environ Microbiol. 2021. PMID: 33103811
However, the molecular determinants of its catabolism are poorly understood. Here, the complete genome of strain JS3050 was sequenced and key genes were expressed heterologously to establish the details of its degradation pathway. ...
However, the molecular determinants of its catabolism are poorly understood. Here, the complete genome of strain JS3050 was sequenced and …
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