Objective: To study the expression of the gene of myosin regulatory light chain-2 (MYL2) in the development of rat testis tissue.
Methods: Using real-time PCR and immunohistochemistry, we determined the mRNA transcription level and protein expression of MYL2 in the rat testis.
Results: The mRNA expression of the MYL2 gene changed in an age-dependent manner, reaching the highest value on postnatal day (PND) 2, then dropped rapidly till PND 8, increased slowly on PNDs 10 and 12, decreased on PND 14, rose slightly from PND 15 and rapidly on PNDs 20 and 25, and declined slowly from PND 65. Immunohistochemistry showed that the MYL2 protein was mainly expressed in testicular sperm cells.
Conclusions: The MYL2 gene may be involved in the proliferation of spermatogonial stem cells and the process of sperm cells developing into mature sperm.
目的： 研究肌球蛋白调节性轻链（MYL2）基因在大鼠睾丸组织发育过程中的表达。方法： 采用RT-PCR、免疫组化分别检测大鼠睾丸组织中MYL2基因的mRNA转录水平和蛋白的表达。 结果： MYL2 mRNA表达在出生后第2天达到最高值，随后迅速下降至第8天，第10、12天缓慢升高，第14天下降，第15天稍有上升，第20、25天出现一个小高峰之后到第65天持续缓慢下降，呈现明显的日龄依赖性。免疫组化结果显示MYL2 蛋白主要表达于睾丸组织精子细胞。结论： MYL2基因可能参与大鼠精原干细胞的增殖， 同时可能参与精子细胞形成成熟精子过程。.
Keywords: rat; spermatogenesis; spermatogonial stem cell; myosin regulatory light chain.