[Effect of Salvia miltiorrhiza on autocrining growth factor by fibroblasts cultured in vitro]

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2002 Nov;16(6):408-10.
[Article in Chinese]

Abstract

Objective: To investigate the mechanism of overhealing alleviation by salvia miltiorrhiza (SM) in wound healing.

Methods: Fibroblasts were cultured in vitro, and SM was applied with different concentrations (40, 80, 160 and 320 micrograms/ml) and time(the 1st, 2nd, 3rd, 4th and 5th days) to influence their autocrine. The levels of transforming growth factor-beta 1 (TGF-beta 1) and epidermal growth factor (EGF) were determined by ELIAS and radioimmunoassay respectively.

Results: The SM could inhibit autocrine of TGF-beta 1 by fibroblasts (P < 0.05). However, it did not affect autocrine of EGF (P > 0.05).

Conclusion: The above results indicate that SM reduces overhealing by inhibiting the autocrine of TGF-beta 1 selectively.

Publication types

  • English Abstract

MeSH terms

  • Cell Division
  • Cells, Cultured
  • Child
  • Cicatrix, Hypertrophic / pathology
  • Collagen / drug effects
  • Drugs, Chinese Herbal / pharmacology*
  • Epidermal Growth Factor / drug effects*
  • Extracellular Matrix / metabolism
  • Fibroblasts / cytology
  • Fibroblasts / drug effects*
  • Humans
  • Salvia miltiorrhiza*
  • Time Factors
  • Transforming Growth Factors / drug effects*
  • Wound Healing

Substances

  • Drugs, Chinese Herbal
  • Epidermal Growth Factor
  • Transforming Growth Factors
  • Collagen