Abstract
Objective:
To investigate the effect of Salvia miltiorrhiza on glomerulosclerosis induced by Ang II.
Method:
Rat mesangial cells were exposed to 100 nmol L(-1) Ang II. Meanwhile, we added S. miltiorrhiza injection of different concentrations to Mcs. PAI-1 mRNA and protein, TGF-beta1 in serum free MEM medium, and the level of cellular reactive oxygen species (ROS) were measured.
Result:
S. miltiorrhiza notably attenuated Ang II induced expression of PAI-1 in a concentration-dependent manner. Meanwhile, S. miltiorrhiza suppressed the production of TGF-beta1 and cellular ROS in mesangial cells.
Conclusion:
S. miltiorrhiza can alleviate glomerular sclerosis. The renoprotective effects of S. miltiorrhiza may be due to its ability to decrease Ang II -induced PAI-1 and TGF-beta1 secretion and cellular ROS level.
MeSH terms
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Angiotensin II / pharmacology
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Animals
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Blotting, Western
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Cells, Cultured
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Dose-Response Relationship, Drug
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Drugs, Chinese Herbal / administration & dosage
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Drugs, Chinese Herbal / isolation & purification
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Drugs, Chinese Herbal / pharmacology*
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Gene Expression / drug effects
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Injections
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Mesangial Cells / drug effects*
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Mesangial Cells / metabolism
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Plants, Medicinal / chemistry
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Plasminogen Activator Inhibitor 1 / biosynthesis*
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Plasminogen Activator Inhibitor 1 / genetics
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Reactive Oxygen Species / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
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Salvia miltiorrhiza / chemistry*
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Transforming Growth Factor beta1 / blood
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Transforming Growth Factor beta1 / metabolism*
Substances
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Drugs, Chinese Herbal
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Plasminogen Activator Inhibitor 1
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RNA, Messenger
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Reactive Oxygen Species
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Transforming Growth Factor beta1
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Angiotensin II