Sensitive poliovirus detection using nested PCR and nanopore sequencing: a prospective validation study.
Shaw AG, Mampuela TK, Lofiko EL, Pratt C, Troman C, Bujaki E, O'Toole Á, Akello JO, Aziza AA, Lusamaki EK, Makangara JC, Akonga M, Lay Y, Nsunda B, White B, Jorgensen D, Pukuta E, Riziki Y, Rankin KE, Rambaut A, Ahuka-Mundeke S, Muyembe JJ, Martin J, Grassly NC, Mbala-Kingebeni P.
Shaw AG, et al.
Nat Microbiol. 2023 Sep;8(9):1634-1640. doi: 10.1038/s41564-023-01453-4. Epub 2023 Aug 17.
Nat Microbiol. 2023.
PMID: 37591995
Free PMC article.
Timely detection of outbreaks is needed for poliovirus eradication, but gold standard detection in the Democratic Republic of the Congo takes 30 days (median). ...Here we report prospective testing of stool samples from suspected polio ca …
Timely detection of outbreaks is needed for poliovirus eradication, but gold standard detection in the Democratic Republic …