DNA Interaction with Coordination Compounds of Cd(II)containing 1,10-Phenanthroline

Int J Mol Sci. 2024 Feb 2;25(3):1820. doi: 10.3390/ijms25031820.

Abstract

The experimental study of the DNA interaction with three cadmium coordination compounds [Cd(phen)3](CH3CO2)2, [Cd(phen)2(H2O)2](CH3CO2)2, and [Cd2(phen)4(H2O)2](CH3CO2)4 was carried out using spectrophotometry, viscosity, and dynamic light scattering methods. The role of the solution ionic strength (concentration of NaCl) was analyzed. All compounds can penetrate (fully or partly) to the major or minor DNA grooves. It was shown that, in addition to the important role of electrostatic interactions in the formation of the complex, intercalation of the 1,10-phenanthroline ligand occurs for compounds [Cd(phen)2(H2O)2](CH3CO2)2 and [Cd2(phen)4(H2O)2](CH3CO2)4. Compound [Cd(phen)3](CH3CO2)2 binds to DNA externally. The coordination bond between cadmium and DNA was formed in DNA complexes with [Cd2(phen)4(H2O)2](CH3CO2)4. Preliminary computer modeling of the DNA interaction with the compounds used was performed.

Keywords: 1,10-phenanthroline ligands; Cd(II) compounds; DNA binding; intercalation; molecular dynamics.

MeSH terms

  • Cadmium
  • Carbon Dioxide
  • Coordination Complexes* / chemistry
  • DNA / chemistry
  • Ligands
  • Organometallic Compounds* / chemistry
  • Phenanthrolines / chemistry
  • Spectrophotometry

Substances

  • Cadmium
  • 1,10-phenanthroline
  • Carbon Dioxide
  • DNA
  • Phenanthrolines
  • Ligands
  • Coordination Complexes
  • Organometallic Compounds

Grants and funding

This research was supported by the Saint Petersburg State University. The synthesis of cadmium compounds was performed at the I.V. Grebenshchikov Institute of Silicate Chemistry of the Russian Academy of Sciences within the framework of the theme of the state budget: “Physico-chemical bases of inorganic synthesis of micro- and nanostructured inorganic, organo-inorganic and ceramic materials and coatings for bio-, energy- and resource-saving technologies” (1021050501070-0-1.4.3, № 0097-2022-0006). Computer simulation was performed using resources provided by “Computer Center of SPbU” (http://www.cc.spbu.ru/en).