Objective: This study aimed to investigate the difference in salivary protease expression in patients with chronic periodontitis and normal individuals.
Methods: The stimulating saliva in patients with chronic periodontitis and normal individuals were collected. Protein chip technology was adapted to analyze salivary protease spectrum.
Results: Among the 34 proteases in the chip, disintegrin and metalloproteinase (ADAM)8, matrix metalloproteinase (MMP)-8, MMP-12, neprilysin/CD10, and uridylyl phosphate adenosine/urokinase showed a significantly increased concentration in the saliva of chronic periodontitis patients compared with those in the saliva of normal individuals (P<0.01). By contrast, the concentrations of ADAM9, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)1, ADAMTS13, cathepsin B, E, L, V, X/Z/P, kallikrein 6, 7, 11, 13, MMP-9, proteinase 3, presenilin-1, and proprotein convertase 9 sharply decreased (P<0.05).
Conclusions: The results demonstrated that protease spectrum in the saliva of chronic periodontitis patients and normal individuals significantly differed. Analysis of salivary protease spectrum is a potential clinical method to examine, diagnose, and monitor chronic periodontitis.
目的 比较慢性牙周炎患者和牙周健康者的唾液蛋白谱差异，以期为慢性牙周炎的诊断和治疗监测提供依据。方法 收集慢性牙周炎患者和牙周健康者刺激性唾液，采用蛋白芯片技术，对慢性牙周炎患者和正常人的唾液蛋白酶谱进行分析。结果 慢性牙周炎患者和牙周健康者唾液蛋白酶的表达具有统计学差异。其中，慢性牙周炎患者唾液中去整合素金属蛋白酶（ADAM）8，基质金属蛋白酶（MMP）-8、-12，脑啡肽酶/CD10，尿激酶纤维蛋白溶酶原激活剂/尿激酶的表达高于牙周健康者（P<0.01）；ADAM9，含凝血酶敏感素基序的去整合素金属蛋白酶（ADAMTS）1、13，组织蛋白酶B、E、L、V、X/Z/P，激肽释放酶6、7、11、13，MMP-9，蛋白酶3、早老素1和前蛋白转化酶9的表达低于牙周健康者（P<0.05）。结论 慢性牙周炎患者的唾液蛋白酶谱与牙周健康者具有显著差异，唾液蛋白酶谱分析将有望成为慢性牙周炎临床诊断和治疗监测的实验检查手段。.
Keywords: chronic periodontitis; protease spectrum; saliva.