The difference in joint instability affects the onset of cartilage degeneration or subchondral bone changes

K Arakawa; K Takahata; S Enomoto; Y Oka; K Ozone; S Nakagaki; K Murata; N Kanemura; T Kokubun

doi:10.1016/j.joca.2021.12.002

The difference in joint instability affects the onset of cartilage degeneration or subchondral bone changes

Osteoarthritis Cartilage. 2022 Mar;30(3):451-460. doi: 10.1016/j.joca.2021.12.002. Epub 2021 Dec 11.

Authors

K Arakawa¹, K Takahata², S Enomoto³, Y Oka⁴, K Ozone⁵, S Nakagaki⁶, K Murata⁷, N Kanemura⁸, T Kokubun⁹

Affiliations

¹ Department of Health and Social Services, Health and Social Services, Graduate School of Saitama Prefectural University, Saitama, Japan. Electronic address: 2391001y@spu.ac.jp.
² Department of Health and Social Services, Health and Social Services, Graduate School of Saitama Prefectural University, Saitama, Japan. Electronic address: 2281308c@spu.ac.jp.
³ Department of Physical Therapy, Health and Social Services, Saitama Prefectural University, Saitama, Japan. Electronic address: 2013005e@spu.ac.jp.
⁴ Department of Health and Social Services, Health and Social Services, Graduate School of Saitama Prefectural University, Saitama, Japan. Electronic address: 2191001s@spu.ac.jp.
⁵ Department of Health and Social Services, Health and Social Services, Graduate School of Saitama Prefectural University, Saitama, Japan; Japan Society for the Promotion of Science, Tokyo, Japan. Electronic address: 2191002a@spu.ac.jp.
⁶ Department of Health and Social Services, Health and Social Services, Graduate School of Saitama Prefectural University, Saitama, Japan. Electronic address: 2181308b@spu.ac.jp.
⁷ Department of Health and Social Services, Health and Social Services, Graduate School of Saitama Prefectural University, Saitama, Japan; Department of Physical Therapy, Health and Social Services, Saitama Prefectural University, Saitama, Japan. Electronic address: murata-kenji@spu.ac.jp.
⁸ Department of Health and Social Services, Health and Social Services, Graduate School of Saitama Prefectural University, Saitama, Japan; Department of Physical Therapy, Health and Social Services, Saitama Prefectural University, Saitama, Japan. Electronic address: kanemura-naohiko@spu.ac.jp.
⁹ Department of Health and Social Services, Health and Social Services, Graduate School of Saitama Prefectural University, Saitama, Japan; Department of Physical Therapy, Health and Social Services, Saitama Prefectural University, Saitama, Japan. Electronic address: kokubun-takanori@spu.ac.jp.

PMID: 34906679
DOI: 10.1016/j.joca.2021.12.002

Abstract

Objective: It has been debated whether the onset of knee osteoarthritis is initiated in cartilage or subchondral bone. The purpose of this study was to clarify the effects of increasing or decreasing joint instability on cartilage degeneration and subchondral bone changes in knee OA by comparing different models of joint instability.

Design: We used the anterior cruciate ligament transection (ACL-T) model and the destabilization of the medial meniscus (DMM) model. In addition, we created a controlled abnormal tibial translation (CATT) model and a controlled abnormal tibial rotation (CATR) model. We performed joint instability analysis, micro-computed tomography analysis, histological and immunohistological analysis in 4 and 6 weeks.

Results: The CATT group suppressed joint instability in the ACL-T group (6 weeks; P = 0.032), and the CATR group suppressed joint instability in the DMM group (6 weeks; P = 0.032). Chondrocyte hypertrophy in the ACL-T and DMM groups was increased compared to the Sham group (6 weeks; [ACL-T vs Sham], P = 0.002, 95%CI [5.983-33.025]; [DMM vs Sham], P = 0.022, 95%CI [1.691-28.733]). In the subchondral bone, the BV/TV in the DMM and CATR groups was increased compared to the ACL-T and CATT groups (6 weeks; [DMM vs ACL-T], P = 0.002, 95%CI [7.404-37.582]; [DMM vs CATT], P = 0.014, 95%CI [2.881-33.059]; [CATR vs ACL-T], P = 0.006, 95%CI [4.615-34.793]; [CATR vs CATT], P = 0.048, 95%CI [0.092-30.270]).

Conclusions: This study showed that joint instability promotes chondrocyte hypertrophy, but subchondral bone changes were influenced by differences in ACL and meniscus function.

Keywords: Joint instability; Osteoarthritis; Pathogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anterior Cruciate Ligament Injuries / complications*
Cartilage Diseases / etiology*
Chondrocytes / pathology
Disease Models, Animal
Joint Instability / complications*
Male
Mice
Osteoarthritis, Knee / etiology*
Tibial Meniscus Injuries / complications*