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Tracking human antigen-specific memory B cells: a sensitive and generalized ELISPOT system.
J Immunol Methods. 2004 Mar;286(1-2):111-22. doi: 10.1016/j.jim.2003.12.015.
J Immunol Methods. 2004.
PMID: 15087226
This assay utilizes a 6-day polyclonal stimulation of PBMC followed by an antigen-specific ELISPOT for the detection of memory B cells that have differentiated into antibody secreting cells (ASC) in vitro. ...Furthermore, these results confirm that vaccine-elicited …
This assay utilizes a 6-day polyclonal stimulation of PBMC followed by an antigen-specific ELISPOT for the detection of memory …
A Cytokine-Independent Approach To Identify Antigen-Specific Human Germinal Center T Follicular Helper Cells and Rare Antigen-Specific CD4+ T Cells in Blood.
Dan JM, Lindestam Arlehamn CS, Weiskopf D, da Silva Antunes R, Havenar-Daughton C, Reiss SM, Brigger M, Bothwell M, Sette A, Crotty S.
Dan JM, et al.
J Immunol. 2016 Aug 1;197(3):983-93. doi: 10.4049/jimmunol.1600318. Epub 2016 Jun 24.
J Immunol. 2016.
PMID: 27342848
Free PMC article.
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