Novel Automation of an Enzyme-Linked Immunosorbent Spot Assay Testing Method: Comparable Diagnostic Performance of the T-SPOT.TB Test Using Manual Density Gradient Cell Isolation versus Automated Positive Selection with the T-Cell Select Kit.
Wang SH, Rajaram MVS, Trollip A, Wu Q, Ayala D, Garza D, Benavidez MA, Fox K, Aguillón-Durán GP, Vargas-Orozco EA, Torres L, Yu L, Ahmed SR, Aspden M, Jackson-Soutter T, Coxon C, Brignall R, Restrepo BI.
Wang SH, et al.
J Clin Microbiol. 2022 Sep 21;60(9):e0055122. doi: 10.1128/jcm.00551-22. Epub 2022 Aug 30.
J Clin Microbiol. 2022.
PMID: 36040150
Free PMC article.
The previous maximum time between blood collection and processing for the T-SPOT.TB test is 32 h using T-Cell Xtend. For this, we compared the T-SPOT.TB test using manual peripheral blood mononuclear cell (PBMC) isolation by density gradient separation at 0 to 8 h (referen …
The previous maximum time between blood collection and processing for the T-SPOT.TB test is 32 h using T-Cell Xtend. For this, we compared t …