[Assessment of the estrogenicity of endosulfan and other chemicals with the in vitro proliferation of human breast cancer cell]

Yan Chang; Xin-Qiang Zhu; Hui-Juan Zhu; Yi-Fan Zheng; Xing-Shu Huang

doi:10.3785/j.issn.1008-9292.2002.04.013

[Assessment of the estrogenicity of endosulfan and other chemicals with the in vitro proliferation of human breast cancer cell]

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2002 Aug;31(4):281-283. doi: 10.3785/j.issn.1008-9292.2002.04.013.

[Article in Chinese]

Authors

Yan Chang¹, Xin-Qiang Zhu, Hui-Juan Zhu, Yi-Fan Zheng, Xing-Shu Huang

Affiliation

¹ School of Public Health, College of Medical Science, Zhejiang University, Hangzhou 310006 China.

PMID: 12601910
DOI: 10.3785/j.issn.1008-9292.2002.04.013

Abstract

OBJECTIVE: To develop a rapid screening method for xenoestrogens and to screen the estrogenicity of some environmental chemicals. METHODS: The E-SCREEN test was developed based on proliferation of human breast cancer cells (MCF-7), and the estrogenicity of diethylstilbestrol, 4-hydrotamoxifen and endosulfan was assessed. RESULTS: The E-SCREEN detected estradiol at very low concentration as 1x10(-13) mol/L. It was found that diethylstilbestrol was a full agonist of estrogen receptor, endosulfan was a partial agonist, while 4 hydrotamoxifen lacked estrogenic effects at this assay. CONCLUSION: The E-SCREEN test is sensitive, rapid, easy to perform and, therefore, suitable for large scale screening for estrogenicity of environmental chemicals.