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DNAzyme-based quantitative loop-mediated isothermal amplification for strain-specific detection of starter kimchi fermented with Leuconostoc mesenteroides WiKim32.
Lee M, Song JH, Shim WB, Chang JY. Lee M, et al. Food Chem. 2020 Dec 15;333:127343. doi: 10.1016/j.foodchem.2020.127343. Epub 2020 Jun 15. Food Chem. 2020. PMID: 32663746
In the presence of the target amplicon, DNAzyme bound to it, resulting in negligible amounts of green product. In contrast, with the addition of hemin and in the absence of the target amplicon, DNAzyme fragments not bound to the target amplicon formed G-quadruplex-hemin …
In the presence of the target amplicon, DNAzyme bound to it, resulting in negligible amounts of green product. In contrast, with the additio …
Detection of ochratoxin A (OTA) in coffee using chemiluminescence resonance energy transfer (CRET) aptasensor.
Jo EJ, Mun H, Kim SJ, Shim WB, Kim MG. Jo EJ, et al. Food Chem. 2016 Mar 1;194:1102-7. doi: 10.1016/j.foodchem.2015.07.152. Epub 2015 Aug 18. Food Chem. 2016. PMID: 26471659
Dabcyl at the end of the OTA aptamer region plays as a quencher in CRET aptasensor. When hemin and OTA are added, the dabcyl-labeled OTA aptamer approaches to the G-quadruplex-hemin complex by formation of the G-quadruplex-OTA complex. The G-quadruplex-hemin …
Dabcyl at the end of the OTA aptamer region plays as a quencher in CRET aptasensor. When hemin and OTA are added, the dabcyl-labeled …
A structure-switchable aptasensor for aflatoxin B1 detection based on assembly of an aptamer/split DNAzyme.
Seok Y, Byun JY, Shim WB, Kim MG. Seok Y, et al. Anal Chim Acta. 2015 Jul 30;886:182-7. doi: 10.1016/j.aca.2015.05.041. Epub 2015 Jul 8. Anal Chim Acta. 2015. PMID: 26320651
An ultrasensitive, colorimetric and homogeneous strategy for aflatoxin B1 (AFB1) detection, which uses a DNA aptamer and two split DNAzyme halves, has been developed. Split halves of a hemin-binding DNAzymes is combined with an AFB1 aptamer to generate a homogeneous colori …
An ultrasensitive, colorimetric and homogeneous strategy for aflatoxin B1 (AFB1) detection, which uses a DNA aptamer and two split DNAzyme h …
Homogeneous assay of target molecules based on chemiluminescence resonance energy transfer (CRET) using DNAzyme-linked aptamers.
Mun H, Jo EJ, Li T, Joung HA, Hong DG, Shim WB, Jung C, Kim MG. Mun H, et al. Biosens Bioelectron. 2014 Aug 15;58:308-13. doi: 10.1016/j.bios.2014.02.008. Epub 2014 Feb 13. Biosens Bioelectron. 2014. PMID: 24658027
A quencher dye was modified at the 3' end of the aptamer sequence. The incorporation of hemin into the G-quadruplex structure of DNAzyme yields an active HRP-like activity that catalyzes luminol to generate a chemiluminescence (CL) signal. ...
A quencher dye was modified at the 3' end of the aptamer sequence. The incorporation of hemin into the G-quadruplex structure of DNAz …
Colorimetric molecular diagnosis of the HIV gag gene using DNAzyme and a complementary DNA-extended primer.
Kim SU, Batule BS, Mun H, Byun JY, Shim WB, Kim MG. Kim SU, et al. Analyst. 2018 Feb 7;143(3):695-699. doi: 10.1039/c7an01520h. Epub 2018 Jan 4. Analyst. 2018. PMID: 29299545
A single-stranded DNAzyme sequence folds into a G-quadruplex structure with hemin and shows strong peroxidase activity. When the complementary strand binds to the DNAzyme sequence, it blocks the formation of the G-quadraduplex structure and loses its peroxidase activity. . …
A single-stranded DNAzyme sequence folds into a G-quadruplex structure with hemin and shows strong peroxidase activity. When the comp …