A new HPLC method has been developed for analysis of Icariine in four Chinese patent medicines. The symmetric peak of Icariine and good resolution were gotten, when ODS was used as analytical column (30 degrees C) MeOH-KH2PO4 (pH = 2.7, adjusted with H3PO4) (57:43) as a mobile phase; the detective wavelength was 268 nm. The contents of Icariine in Bushen-qiangshen-wan, Guiling-ji, Zhuang-gu-guanjie-wan and Qianliening-chongji were 0.105, 0.0995, 0.0566 and 0.313% respectively.