Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Search Page

Filters

My NCBI Filters

Results by year

Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1985 3
1986 1
1987 2
1988 2
1989 5
1990 4
1991 11
1992 20
1993 12
1994 20
1995 15
1996 14
1997 17
1998 31
1999 26
2000 23
2001 30
2002 38
2003 52
2004 66
2005 112
2006 121
2007 133
2008 166
2009 196
2010 234
2011 314
2012 382
2013 447
2014 591
2015 711
2016 756
2017 903
2018 1111
2019 1331
2020 1688
2021 2059
2022 2668
2023 2853
2024 1316

Text availability

Article attribute

Article type

Publication date

Search Results

16,496 results

Results by year

Filters applied: . Clear all
Page 1
RNA-guided human genome engineering via Cas9.
Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. Mali P, et al. Science. 2013 Feb 15;339(6121):823-6. doi: 10.1126/science.1232033. Epub 2013 Jan 3. Science. 2013. PMID: 23287722 Free PMC article.
Here, we engineer the type II bacterial CRISPR system to function with custom guide RNA (gRNA) in human cells. For the endogenous AAVS1 locus, we obtained targeting rates of 10 to 25% in 293T cells, 13 to 8% in K562 cells, and 2 to 4% in induced pluripotent stem cells. ... …
Here, we engineer the type II bacterial CRISPR system to function with custom guide RNA (gRNA) in human cells. For the endogenous AAV …
Machine-learning-guided directed evolution for protein engineering.
Yang KK, Wu Z, Arnold FH. Yang KK, et al. Nat Methods. 2019 Aug;16(8):687-694. doi: 10.1038/s41592-019-0496-6. Epub 2019 Jul 15. Nat Methods. 2019. PMID: 31308553 Review.
Protein engineering through machine-learning-guided directed evolution enables the optimization of protein functions. ...Here we introduce the steps required to build machine-learning sequence-function models and to use those models to guide engineering, making reco …
Protein engineering through machine-learning-guided directed evolution enables the optimization of protein functions. ...Here we intr …
Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems.
Yang LZ, Wang Y, Li SQ, Yao RW, Luan PF, Wu H, Carmichael GG, Chen LL. Yang LZ, et al. Mol Cell. 2019 Dec 19;76(6):981-997.e7. doi: 10.1016/j.molcel.2019.10.024. Epub 2019 Nov 19. Mol Cell. 2019. PMID: 31757757 Free article.
Here, we identify two endonuclease-deficient, single-component programmable RNA-guided and RNA-targeting Cas13 RNases (dCas13s) that allow robust real-time imaging and tracking of RNAs in live cells, even when using single 20- to 27-nt-long guide RNAs. ...
Here, we identify two endonuclease-deficient, single-component programmable RNA-guided and RNA-targeting Cas13 RNases (dCas13s) that …
An engineered hypercompact CRISPR-Cas12f system with boosted gene-editing activity.
Wu T, Liu C, Zou S, Lyu R, Yang B, Yan H, Zhao M, Tang W. Wu T, et al. Nat Chem Biol. 2023 Nov;19(11):1384-1393. doi: 10.1038/s41589-023-01380-9. Epub 2023 Jul 3. Nat Chem Biol. 2023. PMID: 37400536 Free PMC article.
Compact CRISPR-Cas systems offer versatile treatment options for genetic disorders, but their application is often limited by modest gene-editing activity. Here we present enAsCas12f, an engineered RNA-guided DNA endonuclease up to 11.3-fold more potent than its parent pro …
Compact CRISPR-Cas systems offer versatile treatment options for genetic disorders, but their application is often limited by modest gene-ed …
Effect of Natriuretic Peptide-Guided Therapy on Hospitalization or Cardiovascular Mortality in High-Risk Patients With Heart Failure and Reduced Ejection Fraction: A Randomized Clinical Trial.
Felker GM, Anstrom KJ, Adams KF, Ezekowitz JA, Fiuzat M, Houston-Miller N, Januzzi JL Jr, Mark DB, Piña IL, Passmore G, Whellan DJ, Yang H, Cooper LS, Leifer ES, Desvigne-Nickens P, O'Connor CM. Felker GM, et al. JAMA. 2017 Aug 22;318(8):713-720. doi: 10.1001/jama.2017.10565. JAMA. 2017. PMID: 28829876 Free PMC article. Clinical Trial.
DESIGN, SETTINGS, AND PARTICIPANTS: The Guiding Evidence Based Therapy Using Biomarker Intensified Treatment in Heart Failure (GUIDE-IT) study was a randomized multicenter clinical trial conducted between January 16, 2013, and September 20, 2016, at 45 clinical site …
DESIGN, SETTINGS, AND PARTICIPANTS: The Guiding Evidence Based Therapy Using Biomarker Intensified Treatment in Heart Failure (GUI …
Photoacoustic image-guided interventions.
Karthikesh MS, Yang X. Karthikesh MS, et al. Exp Biol Med (Maywood). 2020 Feb;245(4):330-341. doi: 10.1177/1535370219889323. Epub 2019 Nov 20. Exp Biol Med (Maywood). 2020. PMID: 31747782 Free PMC article. Review.
These studies have demonstrated that photoacoustic imaging can guide these interventions effectively and non-invasively in real-time. ...This is substantiated in this article which sums up the current state and underlines promising results of research using photoacoustic i …
These studies have demonstrated that photoacoustic imaging can guide these interventions effectively and non-invasively in real-time. …
Radiomics and dosiomics for predicting radiation-induced hypothyroidism and guiding intensity-modulated radiotherapy.
Yang SS, Peng QH, Wu AQ, Zhang BY, Liu ZQ, Chen EN, Xie FY, OuYang PY, Chen CY. Yang SS, et al. iScience. 2023 Nov 4;26(12):108394. doi: 10.1016/j.isci.2023.108394. eCollection 2023 Dec 15. iScience. 2023. PMID: 38047064 Free PMC article.
To guide individualized intensity-modulated radiotherapy (IMRT), we developed and prospectively validated a multiview radiomics risk model for predicting radiation-induced hypothyroidism in patients with nasopharyngeal carcinoma. ...The multiview radiomics risk model was a …
To guide individualized intensity-modulated radiotherapy (IMRT), we developed and prospectively validated a multiview radiomics risk …
Expert consensus on digital guided therapy for endodontic diseases.
Wei X, Du Y, Zhou X, Yue L, Yu Q, Hou B, Chen Z, Liang J, Chen W, Qiu L, Huang X, Meng L, Huang D, Wang X, Tian Y, Tang Z, Zhang Q, Miao L, Zhao J, Yang D, Yang J, Ling J. Wei X, et al. Int J Oral Sci. 2023 Dec 6;15(1):54. doi: 10.1038/s41368-023-00261-0. Int J Oral Sci. 2023. PMID: 38052782 Free PMC article. Review.
Digital guided therapy (DGT) has been advocated as a contemporary computer-aided technique for treating endodontic diseases in recent decades. The concept of DGT for endodontic diseases is categorized into static guided endodontics (SGE), necessitating a meticulousl …
Digital guided therapy (DGT) has been advocated as a contemporary computer-aided technique for treating endodontic diseases in recent …
Intraoperative Ultrasound for Peripheral Nerve Applications.
Willsey M, Wilson TJ, Henning PT, Yang LJ. Willsey M, et al. Neurosurg Clin N Am. 2017 Oct;28(4):623-632. doi: 10.1016/j.nec.2017.05.013. Epub 2017 Aug 10. Neurosurg Clin N Am. 2017. PMID: 28917290 Review.
Although intraoperative peripheral nerve localization has been performed previously using guide wires and surgical dyes, the authors' approach using ultrasound-guided instrument clamps helps guide surgical dissection to the target nerve, which could lead to m …
Although intraoperative peripheral nerve localization has been performed previously using guide wires and surgical dyes, the authors' …
[Application of PET-LINAC in Biology-guided Radiotherapy].
Yang X, Zhao W, Tian X, Cai J, Xie S, Liu Q, Peng H, Peng Q. Yang X, et al. Zhongguo Yi Liao Qi Xie Za Zhi. 2023 May 30;47(3):237-241. doi: 10.3969/j.issn.1671-7104.2023.03.001. Zhongguo Yi Liao Qi Xie Za Zhi. 2023. PMID: 37288620 Review. Chinese.
Biology-guided radiotherapy (BgRT) is a novel technique of external beam radiotherapy, combining positron emission tomography-computed tomography (PET-CT) with a linear accelerator (LINAC). ...RefleXion Medical has developed the world's first BgRT system. Nevertheless, its …
Biology-guided radiotherapy (BgRT) is a novel technique of external beam radiotherapy, combining positron emission tomography-compute …
16,496 results
You have reached the last available page of results. Please see the User Guide for more information.