Mind the gap: Micro-expansion joints drastically decrease the bending of FIB-milled cryo-lamellae.
Wolff G, Limpens RWAL, Zheng S, Snijder EJ, Agard DA, Koster AJ, Bárcena M.
Wolff G, et al.
J Struct Biol. 2019 Dec 1;208(3):107389. doi: 10.1016/j.jsb.2019.09.006. Epub 2019 Sep 16.
J Struct Biol. 2019.
PMID: 31536774
Free article.
The severity of lamella bending seems to correlate with crinkling of the EM grid support film at cryogenic temperatures, which could generate tensions that may be transferred onto the thin lamella, leading to its bending and breakage. ...
The severity of lamella bending seems to correlate with crinkling of the EM grid support film at cryogenic temperatures, which could …