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A reliable strategy for single-cell RNA sequencing analysis using cryoconserved primary cortical cells.
J Neurosci Methods. 2021 Jan 1;347:108960. doi: 10.1016/j.jneumeth.2020.108960. Epub 2020 Sep 25.
J Neurosci Methods. 2021.
PMID: 32987100
Free article.
In order to freeze cortical primary cells, we have employed Neurostore, a medium able to guarantee high viability and cell composition of embryonic cortex after thawing. COMPARISON WITH EXISTING METHODS: We showed for the first time the possibility to run scRNASeq experime …
In order to freeze cortical primary cells, we have employed Neurostore, a medium able to guarantee high viability and cell composition of em …
Systematic parameter optimization of a Me(2)SO- and serum-free cryopreservation protocol for human mesenchymal stem cells.
Freimark D, Sehl C, Weber C, Hudel K, Czermak P, Hofmann N, Spindler R, Glasmacher B.
Freimark D, et al.
Cryobiology. 2011 Oct;63(2):67-75. doi: 10.1016/j.cryobiol.2011.05.002. Epub 2011 May 17.
Cryobiology. 2011.
PMID: 21620818
In most cases, cryomicroscopy revealed intracellular ice formation (IIF) during the cryopreservation cycle for all cryoprotocols. The occurence of IIF during thawing increased with the cooling rate. In case of hMSC there was no correlation between the rate of IIF and the p …
In most cases, cryomicroscopy revealed intracellular ice formation (IIF) during the cryopreservation cycle for all cryoprotocols. The occure …
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