Speech variation of speakers is a crucial issue for speaker recognition and identification, especially for forensic practice. Greater intra-speaker variation is one main reason for incorrect speaker identification in real forensic situations. Understanding the stability of acoustic parameters and their variation in speech is therefore significant for the evaluation of effective parameters for speaker identification. In this paper, all vowels in Standard Chinese including five monophthongs, eight diphthongs and four triphthongs were combined with lateral /l/. Finally, 15 lateral syllables with different tones for 10 speakers were selected and acoustically analyzed. Central frequencies of the first four formants for each syllable were measured for quantitative comparison of intra- and inter-speaker variation in order to provide a general idea of speaker variation in Standard Chinese, and finally serving for forensic application. Results show that the overall intra-speaker variation is less than the inter-speaker variation in great extent under laboratory condition though occasionally they are contrary. This supports the basis for forensic speaker identification, that is, intra-speaker variation should be less than inter-speaker variation in many acoustic features, and further validates the probability and reliability of forensic speaker identification.