Objective: To investigate the effects of human amniotic mesenchymal stem cell（AMSC） on acute graft-versus-host disease (aGVHD) in xenotransplatation.
Methods: NPG mice were injected with human PBMNC via tail vein to establish a xenografted aGVHD model. The mice in the experimental group were divided into PBMNC infusion group and PBMNC+AMSC co-infusion group, the general condition, survival time and manifestations of aGVHD were observed, the body weight and blood routine indicators were detected, the pathological changes of aGVHD target organs (lung, liver, spleen, small intestine) were observed by HE staining, and the levels of human T cells in peripheral blood, tissues and organs of mice was detected by flow cytometry.
Results: The manifestations of aGVHD (lassitude hunchback, shrub, weight reduction, etc.) and the pathological damage of the target organs (lung, liver, spleen, intestine) in PBMNC+AMSC co-infusion group were lighter than those in PBMNC infusion group. Moreover, the PBMNC and AMSC co-infusion significantly reduced the implantion proportion of human T lymphocytes (CD3+, CD45+) in mice and increased the ratio of CD4+/CD8+.
Conclusion: Infusion of human-derived AMSC can attenuate the manifestations of aGVHD in mouse xenografts to a certain level, and improve the pathological damage of receptor target organs.
题目: 人羊膜间充质干细胞对异种移植性急性移植物抗宿主病的作用.
目的: 探讨人羊膜间充质干细胞（amniotic mesenchymal stem cell, AMSC）对异种移植的急性移植物抗宿主病（acute graft-versus-host disease，aGVHD）的作用.
方法: 应用NPG（NOD-Prkdcscid Il2rgnull，严重联合免疫缺陷）小鼠通过尾静脉注射人外周血单个核细胞（peripheral blood mononuclear cell，PBMNC），建立异种移植的aGVHD模型。实验组小鼠分为PBMNC输注组和PBMNC+AMSC共输注组，观察各组小鼠的一般情况、生存期、aGVHD表现、体重及血象变化，应用HE染色观察aGVHD靶器官（肺、肝脏、脾脏、小肠）的病理学改变；采用流式细胞术检测人T细胞在小鼠外周血、组织及器官中的植入水平.
结果: PBMNC+AMSC共输注组小鼠的aGVHD表现（倦怠、弓背、耸毛、体重减轻等）及靶器官（肺、肝脏、脾脏、肠）的病理损伤均轻于PBMNC输注组小鼠，且PBMNC+AMSC共输注明显降低了小鼠体内人源T淋巴细胞（CD3+、CD45+）植入比例，提高了CD4+/CD8+比值.
结论: 输注人源AMSC能够明显减轻小鼠异种移植的aGVHD表现，改善受体靶器官的病理损伤.