A phytoextract mediated synthesis of iron oxide nanoparticles using Agrewia optiva (Dhaman or Biul) and Prunus persica (Peach) leaf extract as capping and stabilizing agent without using hazardous toxic chemicals via biogenic route has been studied. The biogenic method of synthesis is convenient, rapid, cost effective and ecofriendly. The green synthesized nanoparticles were characterized by Ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, Attenuated total reflectance spectroscopy, X-ray diffraction analysis, scanning electron microscopy, energy dispersive X-ray spectroscopy, transmission electron microscopy and dynamic light scattering measurements. The antibacterial study was determined by agar well diffusion method to measure the efficiency of both phyto species extract and its mediated iron oxide nanoparticles against five gram positive bacterial stains such as Staphylococcus aureus (S. aureus), Streptococcus mutans (S. mutans), Streptococcus pyrogenes (S. pyrogenes), Corynebacterium diphtheriae (C. diphtheriae) and Corynebacterium xerosis (C. xerosis) and three gram negative bacterial stains such as Escherichia coli (E. coli), Klebsiella pneuomoniae (K. pneuomoniae) and Pseudomonas aeruginosa (P. aeruginosa). The antibiotic Ciprofloxacin and Gentamicin have been used as reference standard drugs for gram positive and gram negative bacterial stains, respectively. The antioxidant activity of the phyto extracts and prepared nanoparticles have been performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay employing l-ascorbic acid as a standard.
Keywords: Antibacterial; Antioxidant activity.; Biogenic; Dynamic light scattering; Iron oxide nanoparticles.
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