Determination of creatine, creatinine, free amino acid and heterocyclic aromatic amine contents of plain beef and chicken juices

J Food Sci Technol. 2021 Sep;58(9):3293-3302. doi: 10.1007/s13197-020-04875-8. Epub 2020 Nov 18.

Abstract

In the present study, creatine, creatinine, free amino acid and heterocyclic aromatic amine (HAA) contents of plain beef and chicken juices were investigated. For this aim, the boned and boneless beef and chicken juices were obtained by boiling for 3 h at 100 °C without any additives in the laboratory. Creatine and creatinine content of the beef juices varied between 1.33-3.16 mg g-1 and 1.29-1.42 mg g-1, respectively, while creatine and creatinine content of the chicken juices varied between 0.98-1.63 mg g-1 and 0.89-1.30 mg g-1, respectively. The total free amino acid content in the beef juices and the chicken juices ranged between 14.61-20.65 mg 100 gdm-1 and 19.66-57.88 mg 100 gdm-1, respectively. None of the heterocyclic aromatic amines analyzed in the present study could be detected in the beef juices and the chicken juices. Therefore, the boned and boneless beef and chicken juice can be considered as safe from the standpoint of these HAAs and are suggested to use in the preparation of meals.

Keywords: Beef juice; Chicken juice; Creatine; Creatinine; Free amino acid content; Heterocyclic aromatic amine.