Identification and Functional Analysis of ToBPI1/LBP and ToBPI2/LBP in Anti-Bacterial Infection of Trachinotus ovatus

Ze-Chang Bian; Xiao-Hui Cai; Kian Ann Tan; Ya-Dan Wang; Zhuang Huang; Kit Yue Kwan; Peng Xu

doi:10.3390/genes14040826

Identification and Functional Analysis of ToBPI1/LBP and ToBPI2/LBP in Anti-Bacterial Infection of Trachinotus ovatus

Genes (Basel). 2023 Mar 29;14(4):826. doi: 10.3390/genes14040826.

Authors

Ze-Chang Bian^{1

2}, Xiao-Hui Cai¹, Kian Ann Tan¹, Ya-Dan Wang¹, Zhuang Huang¹, Kit Yue Kwan¹, Peng Xu¹

Affiliations

¹ Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou 535011, China.
² State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning 530004, China.

Abstract

Bactericidal/permeability-increasing protein (BPI) and lipopolysaccharide-binding protein (LBP) are a group of antibacterial proteins that play an important role in the host's innate immune defense against pathogen infection. In this study, two BPI/LBPs, named ToBPI1/LBP (1434 bp in length, 478 amino acids) and ToBPI2/LBP (1422 bp in length, 474 amino acids), were identified from the golden pompano. ToBPI1/LBP and ToBPI2/LBP were significantly expressed in immune-related tissues after being challenged with Streptococcus agalactiae and Vibrio alginolyticus. The two BPI/LBPs showed significant antibacterial activity against Gram-negative Escherichia coli and Gram-positive S. agalactiae and Streptococcus iniae. In contrast, the antibacterial activity against Staphylococcus aureus, Corynebacterium glutamicum, Vibrio parahaemolyticus, V. alginolyticus and Vibrio harveyi was low and decreased with time. The membrane permeability of bacteria treated with recombinant ToBPI1/LBP and ToBPI2/LBP was significantly enhanced. These results suggest that ToBPI1/LBP and ToBPI2/LBP may play important immunological roles in the immune response of the golden pompano to bacteria. This study will provide basic information and new insights into the immune response mechanism of the golden pompano to bacteria and the function of BPI/LBP.

Keywords: Trachinotus ovatus; bactericidal/permeability-increasing protein; bacteriostatic ability; gene expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Anti-Bacterial Agents
Bacterial Infections*
Base Sequence
Fishes* / genetics
Phylogeny

Substances

lipopolysaccharide-binding protein
Anti-Bacterial Agents

Grants and funding

This work was sponsored by the Science and Technology Base and Talent Project of Guangxi Province (Gui Ke AD19245059), Natural Science Foundation of Guangxi Province (2019GXNSFBA245062), High-level Talents Scientific Research Start-Up Fund Project of Beibu Gulf University (2019KYQD15), Guangxi Middle-aged and Young Teachers’ Basic Ability Promotion Project (2019KY0464), Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation (2021ZC02), ASEAN Talent Program (ATYSP2022008), and Marine Science Program for Guangxi First-Class Discipline, Beibu Gulf University (TRA001, DRA002, ESB010).