[Identification of the triploid hybrid chromosomes of Elymus canadensis L. x Hordeum brivisubulatum Link. by genomic in situ hybridization]

Yi Chuan Xue Bao. 2004 Jul;31(7):735-9.
[Article in Chinese]

Abstract

AThe intergeneric F1 hybrid between Elymus canadensis L. and Hordeum brivisubulatum Link. is a triploid (2n = 3x = 21 ), in which 7 chromosomes,that is equal to the base number of parent's chromosomes, were lost. In order to indentify the chromosome constitution of the triploid F1 hybrid of E. canadensis L. x H. brivisubulatum Link., the genomic in situ hybridization of F1 root tip cell chromosome DNA that H. brivisubulatum H1 H1 H2H2 genome DNA labeled with Biotin-16-dUTP was conducted using E. canadensis SSH(c)H(c) genome as blocking DNA. The result showed that the chromosomes of triploid F1 hybrid consisted of 7 chromosomes from E. canadensis S genome and 14 chromosomes from H. brivisubulatum H1 H2 genome while the 7 chromosomes of Hc genome of E. canadensis were lost.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chimera
  • Chromosomes, Plant*
  • Elymus / genetics*
  • Genome, Plant*
  • Hordeum / genetics*
  • In Situ Hybridization / methods*
  • Polyploidy