Characterization of Mesorhizobium loti L-rhamnose isomerase and its application to L-talose production

Goro Takata; Keiko Uechi; Eriko Taniguchi; Yuka Kanbara; Akihide Yoshihara; Kenji Morimoto; Ken Izumori

doi:10.1271/bbb.110018

Characterization of Mesorhizobium loti L-rhamnose isomerase and its application to L-talose production

Biosci Biotechnol Biochem. 2011;75(5):1006-9. doi: 10.1271/bbb.110018. Epub 2011 May 20.

Authors

Goro Takata¹, Keiko Uechi, Eriko Taniguchi, Yuka Kanbara, Akihide Yoshihara, Kenji Morimoto, Ken Izumori

Affiliation

¹ Rare Sugar Research Center, Kagawa University, Kagawa, Japan. goro@ag.kagawa-u.ac.jp

PMID: 21597169
DOI: 10.1271/bbb.110018

Abstract

The L-rhamnose isomerase gene (rhi) of Mesorhizobium loti was cloned and expressed in Escherichia coli, and then characterized. The enzyme exhibited activity with respect to various aldoses, including D-allose and L-talose. Application of it in L-talose production from galactitol was achieved by a two-step reaction, indicating that it can be utilized in the large-scale production of L-talose.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aldose-Ketose Isomerases / genetics
Aldose-Ketose Isomerases / isolation & purification
Aldose-Ketose Isomerases / metabolism*
Alphaproteobacteria / enzymology*
Cloning, Molecular
Escherichia coli / genetics
Kinetics
Lactones / metabolism*

Substances

L-talo-gamma-lactone
Lactones
Aldose-Ketose Isomerases
L-rhamnose isomerase