Physicochemical properties and protective effects on UVA-induced photoaging in Hs68 cells of Pleurotus ostreatus polysaccharides by fractional precipitation

Yafang Hsiao; Yichia Shao; Yunting Wu; Wenkuang Hsu; Kuanchen Cheng; Chengchia Yu; Chunhsu Chou; Changwei Hsieh

doi:10.1016/j.ijbiomac.2022.12.254

Physicochemical properties and protective effects on UVA-induced photoaging in Hs68 cells of Pleurotus ostreatus polysaccharides by fractional precipitation

Int J Biol Macromol. 2023 Feb 15:228:537-547. doi: 10.1016/j.ijbiomac.2022.12.254. Epub 2022 Dec 27.

Authors

Yafang Hsiao¹, Yichia Shao², Yunting Wu³, Wenkuang Hsu⁴, Kuanchen Cheng⁵, Chengchia Yu⁶, Chunhsu Chou⁷, Changwei Hsieh⁸

Affiliations

¹ Department of Food Science and Biotechnology, National Chung Hsing University, No. 145, Xingda Rd., South Dist., Taichung City 402202, Taiwan. Electronic address: evo1031nne@gmail.com.
² Department of Food Science and Biotechnology, National Chung Hsing University, No. 145, Xingda Rd., South Dist., Taichung City 402202, Taiwan. Electronic address: a14253681@gmail.com.
³ Department of Food Science and Biotechnology, National Chung Hsing University, No. 145, Xingda Rd., South Dist., Taichung City 402202, Taiwan. Electronic address: qwa56jt7@gmail.com.
⁴ Department of Medicinal Botanicals and Foods on Health Applications, Da-Yeh University, No. 168, Xuefu Rd., Dacun Township, Changhua County 515006, Taiwan. Electronic address: andyhsu@mail.dyu.edu.tw.
⁵ Institute of Biotechnology, National Taiwan University, No. 81, Changxing St., Da'an Dist., Taipei City 106038, Taiwan; Institute of Food Science and Technology, National Taiwan University, No. 59, Ln. 144, Sec. 4, Keelung Rd., Da'an Dist., Taipei City 106032, Taiwan; Department of Optometry, Asia University, No.500, Liufeng Rd., Wufeng Dist., Taichung City 413305, Taiwan; Department of Medical Research, China Medical University Hospital, No. 2, Yude Rd., North Dist., Taichung City 404327, Taiwan. Electronic address: kccheng@ntu.edu.tw.
⁶ Institute of Oral medicine, Chung Shan Medical University, No. 110, Sec. 1, Jianguo N. Rd., South Dist., Taichung City 402306, Taiwan. Electronic address: ccyu@csmu.edu.tw.
⁷ Dr Jou Biotech Co., Ltd., No. 21, Lugong S. 2nd Rd., Lukang Township, Changhua County 505029, Taiwan. Electronic address: dr.jason.jou@gmail.com.
⁸ Department of Food Science and Biotechnology, National Chung Hsing University, No. 145, Xingda Rd., South Dist., Taichung City 402202, Taiwan; Department of Medical Research, China Medical University Hospital, No. 2, Yude Rd., North Dist., Taichung City 404327, Taiwan. Electronic address: welson@nchu.edu.tw.

PMID: 36584774
DOI: 10.1016/j.ijbiomac.2022.12.254

Abstract

The development of natural ingredients protecting skin from UVA-induced photoaging is widely expected. The present study investigated the physicochemical properties, antioxidant, moisturizing, collagenase and elastase inhibitory activities, and protective effect on UVA-induced photoaging in Hs68 cells of Pleurotus ostreatus polysaccharides (POPs). POP-40, POP-60, and POP-80 were extracted by gradient precipitation of 40 %, 60 %, and 80 % ethanol, which could be prepared in large quantities. The results showed that POPs had good DPPH and ABTS radical scavenging abilities, water retention capacity, and collagenase and elastase inhibition effects. POP-80 had the best efficacy. Further determined the anti-inflammatory and antisenescence activities of POPs in Hs68 cells. The results indicated that after UVA irradiation, the contents of ROS, senescent cells, NF-κB activity, and proinflammatory cytokines increased in Hs68 cells. However, cells pretreated with 50 μg/mL POPs significantly decreased the contents of ROS and the number of senescent cells, reduced NF-κB activity, and inhibited IL-6 and TNF-α production. There was no significant difference in reducing the accumulation of ROS and senescent cells between POP-80 and the common anti-inflammatory substance quercetin. The results suggested that POP-80 may be potential cosmeceutical ingredients as it can protect Hs68 cells from photodamage.

Keywords: Photoaging; Pleurotus ostreatus; Polysaccharide.

MeSH terms

Anti-Inflammatory Agents / pharmacology
Fractional Precipitation
Humans
NF-kappa B
Pleurotus* / chemistry
Polysaccharides / chemistry
Polysaccharides / pharmacology
Reactive Oxygen Species
Skin Aging*
Skin Diseases*
Ultraviolet Rays / adverse effects

Substances

Anti-Inflammatory Agents
NF-kappa B
Polysaccharides
Reactive Oxygen Species