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Fusion crystallization reveals the behavior of both the 1TEL crystallization chaperone and the TNK1 UBA domain.
Nawarathnage S, Tseng YJ, Soleimani S, Smith T, Pedroza Romo MJ, Abiodun WO, Egbert CM, Madhusanka D, Bunn D, Woods B, Tsubaki E, Stewart C, Brown S, Doukov T, Andersen JL, Moody JD. Nawarathnage S, et al. Among authors: tseng yj. Structure. 2023 Dec 7;31(12):1589-1603.e6. doi: 10.1016/j.str.2023.09.001. Epub 2023 Sep 29. Structure. 2023. PMID: 37776857 Free article.
Micrococcin cysteine-to-thiazole conversion through transient interactions between a scaffolding protein and two modification enzymes.
Calvopina-Chavez DG, Bursey DM, Tseng YJ, Patil LM, Bewley KD, Bennallack PR, McPhie JM, Wagstaff KB, Daley A, Miller SM, Moody JD, Price JC, Griffitts JS. Calvopina-Chavez DG, et al. Among authors: tseng yj. bioRxiv [Preprint]. 2023 Oct 23:2023.10.23.563616. doi: 10.1101/2023.10.23.563616. bioRxiv. 2023. PMID: 37961320 Free PMC article. Preprint.
Fusion crystallization reveals the behavior of both the 1TEL crystallization chaperone and the TNK1 UBA domain.
Nawarathnage S, Tseng YJ, Soleimani S, Smith T, Romo MJP, Abiodun WO, Egbert CM, Madhusanka D, Bunn D, Woods B, Tsubaki E, Stewart C, Brown S, Doukov T, Andersen JL, Moody JD. Nawarathnage S, et al. Among authors: tseng yj. bioRxiv [Preprint]. 2023 Jun 14:2023.06.14.544429. doi: 10.1101/2023.06.14.544429. bioRxiv. 2023. PMID: 37398013 Free PMC article. Updated. Preprint.
Increasing the bulk of the 1TEL-target linker and retaining the 10×His tag in a 1TEL-CMG2-vWa construct improves crystal order and diffraction limits.
Gajjar PL, Pedroza Romo MJ, Litchfield CM, Callahan M, Redd N, Nawarathnage S, Soleimani S, Averett J, Wilson E, Lewis A, Stewart C, Tseng YJ, Doukov T, Lebedev A, Moody JD. Gajjar PL, et al. Among authors: tseng yj. Acta Crystallogr D Struct Biol. 2023 Oct 1;79(Pt 10):925-943. doi: 10.1107/S2059798323007246. Epub 2023 Sep 25. Acta Crystallogr D Struct Biol. 2023. PMID: 37747038 Free PMC article.
Decreasing the flexibility of the TELSAM-target protein linker and omitting the cleavable fusion tag improves crystal order and diffraction limits.
Gajjar PL, Romo MJP, Litchfield CM, Callahan M, Redd N, Nawarathnage S, Soleimani S, Averett J, Wilson E, Lewis A, Stewart C, Tseng YJ, Doukov T, Lebedev A, Moody JD. Gajjar PL, et al. bioRxiv [Preprint]. 2023 May 15:2023.05.12.540586. doi: 10.1101/2023.05.12.540586. bioRxiv. 2023. PMID: 37293010 Free PMC article. Preprint.