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Cloning, expression, purification, and characterization of a novel epoxide hydrolase from Aspergillus niger SQ-6.
Liu Y, Wu S, Wang J, Yang L, Sun W. Liu Y, et al. Protein Expr Purif. 2007 Jun;53(2):239-46. doi: 10.1016/j.pep.2006.06.017. Epub 2006 Jun 27. Protein Expr Purif. 2007. PMID: 17317214
The electrophoretic pure recombinant protein, which shows similar characterization as natural enzyme purified from A. niger SQ-6, can be easily purified by Ni(2+)-chelated affinity and gel-filtration chromatography. Optimal pH and temperature for purified enzyme are pH 7.5 …
The electrophoretic pure recombinant protein, which shows similar characterization as natural enzyme purified from A. niger SQ-6, can be eas …