Objective: We studied the ability of Lactobacillus plantarum ZJ8 to bind fumonisins FB1 and FB2.
Methods: The percentage of FB1 and FB2 bound by the strain was measured by HPLC after bacterial cells and FB1 and FB2 were co-incubated in MRS media at 37% for 4 h.
Results: The percentage of FB1- and FB2-binding was 89.9% and 95.0%, respectively. The FBs-binding rate of strain ZJ8 reached the maximum at pH 4. Binding of FBs was poor under alkaline conditions and high temperatures. After acid and SDS treatment, the FBs-binding rate significantly increased. The percentage of FB1- and FB2-binding was 96.8% and 100% for the cell walls of strain ZJ8, respectively. Moreover, about 96.8% FB, and 100% FB, was bound to the peptidoglycan of strain ZJ8' s cell walls.
Conclusions: L. plantarum ZJ8 has potential to remove fumonisins. The peptidoglycan of cell walls from strain ZJ8 was proved to be the main site of FBs-binding.