Comparative transcriptome analyses reveal the dynamic responses of avian myotubes to acute heat stress

Dongdong Yang; Xudong Zhu; Zhen Liu; Xiaofei Wang; Lin Zhang; Tong Xing; Feng Gao

doi:10.1016/j.jtherbio.2022.103235

Comparative transcriptome analyses reveal the dynamic responses of avian myotubes to acute heat stress

J Therm Biol. 2022 May:106:103235. doi: 10.1016/j.jtherbio.2022.103235. Epub 2022 Mar 29.

Authors

Dongdong Yang¹, Xudong Zhu², Zhen Liu¹, Xiaofei Wang², Lin Zhang¹, Tong Xing³, Feng Gao⁴

Affiliations

¹ College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Joint International Research Laboratory of Animal Health and Food Safety, National Experimental Teaching Demonstration Center of Animal Science, Nanjing Agricultural University, Nanjing, 210095, PR China.
² College of Science, Nanjing Agricultural University, Nanjing, China.
³ College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Joint International Research Laboratory of Animal Health and Food Safety, National Experimental Teaching Demonstration Center of Animal Science, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: xingtong@njau.edu.cn.
⁴ College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Joint International Research Laboratory of Animal Health and Food Safety, National Experimental Teaching Demonstration Center of Animal Science, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: gaofeng0629@sina.com.

PMID: 35636894
DOI: 10.1016/j.jtherbio.2022.103235

Abstract

The detrimental effects of acute heat stress (AHS) on poultry production have been widely reported. Unraveling the physiological and metabolic responses to AHS could help to provide theoretical basis for developing strategies to mitigate hyperthermia-induced muscle damage. Here, we investigated the effects of different durations of AHS (45 °C for 0.5, 1, 2 and 4 h) on differentiated avian myotubes. Results indicated that AHS destroyed the morphology of differentiated myotubes, and the degree of damage increased with the prolongation of AHS. Dynamic transcriptomic profiling identified 67, 467, 1355 and 2627 differentially expressed genes (DEGs) after 0.5, 1, 2, and 4 h of heat stress, respectively. Only 50 DEGs were regulated across all time points. In addition, genes involved in cell cycle, metabolic process and immune response were upregulated upon short-term heat stress (0.5 and 1 h). However, these thermal-tolerance responses were suppressed upon prolonged heat stress (2 and 4 h). Furthermore, the quick response of molecular chaperone genes might be major targets for acclimation to hyperthermia. Overall, the current transcriptome analyses reveal the dynamic changes of avian myotubes to AHS and promote an understanding of the molecular mechanisms involved in the heat stress response in poultry.

Keywords: Acute heat stress; Heat shock protein; Myotube; Transcriptome.

MeSH terms

Animals
Birds
Gene Expression Profiling / methods
Heat-Shock Response* / genetics
Muscle Fibers, Skeletal
Transcriptome*