Photoacoustic probe of targeting intracellular Staphylococcus aureus infection with signal-enhanced by self-assembly

Xin Jin; Yue Fei; Jingmei Ma; Li-Li Li; Hao Wang

doi:10.1016/bs.mie.2021.06.027

Photoacoustic probe of targeting intracellular Staphylococcus aureus infection with signal-enhanced by self-assembly

Methods Enzymol. 2021:657:331-347. doi: 10.1016/bs.mie.2021.06.027. Epub 2021 Jul 19.

Authors

Xin Jin¹, Yue Fei¹, Jingmei Ma¹, Li-Li Li¹, Hao Wang²

Affiliations

¹ CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), University of Chinese Academy of Sciences, Beijing, China.
² CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), University of Chinese Academy of Sciences, Beijing, China. Electronic address: wanghao@nanoctr.cn.

PMID: 34353493
DOI: 10.1016/bs.mie.2021.06.027

Abstract

In this chapter, we introduce the photoacoustic imaging probe in detecting the infection of bacterial in host cells. We give detailed protocols for the synthesis and confirmation of a PA probe which response to caspase-1 and then self-assembly to enhance the signal. With this PA contrast agent, we may provide a new approach for an intracellular bacterial infection with the selective and sensitive diagnosis.

Keywords: Intracellular infection; Photoacoustic probe; Self-assembly.

MeSH terms

Contrast Media
Humans
Photoacoustic Techniques*
Spectrum Analysis
Staphylococcal Infections*
Staphylococcus aureus

Substances

Contrast Media