Magnetic Dynabeads detection by sensitive element based on giant magnetoimpedance

Biosens Bioelectron. 2005 Feb 15;20(8):1611-6. doi: 10.1016/j.bios.2004.04.027.

Abstract

A GMI-biosensor prototype was designed and fabricated using an amorphous ribbon. As a first step, a GMI response was measured with a model liquid media: suspension containing magnetisable Dynabeads M-450. These beads can be used as biomolecular labels when coated with a specific antibody. The GMI change caused by their presence was measured with a biosensor prototype designed with a measuring cell containing an amorphous CoFeMoSiB-ribbon element. GMI was measured at a range of current frequencies from 0.3 to 10 MHz and at intensities of either Irms=3 or 4.5 mA. Commercial Dynabeads M-450 were supplied as a suspension in a phosphate buffered saline. A maximum difference of 25% in the GMI ratio measured with and without Dynabeads was obtained at a frequency of 3.5 MHz and Irms=3 mA. Some potential applications for GMI-biosensor and further directions are proposed.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Electric Impedance*
  • Electrochemistry / instrumentation*
  • Electrochemistry / methods
  • Equipment Design
  • Equipment Failure Analysis
  • Immunoassay / instrumentation*
  • Immunoassay / methods
  • Magnetics*
  • Microspheres*
  • Molecular Probe Techniques / instrumentation*
  • Reproducibility of Results
  • Sensitivity and Specificity