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PCR incorporation of dUMPs modified with aromatic hydrocarbon substituents of different hydrophilicities: Synthesis of C5-modified dUTPs and PCR studies using Taq, Tth, Vent (exo-) and Deep Vent (exo-) polymerases.
Zasedateleva OA, Surzhikov SA, Shershov VE, Miftakhov RA, Yurasov DA, Kuznetsova VE, Chudinov AV. Zasedateleva OA, et al. Among authors: kuznetsova ve. Bioorg Chem. 2020 Jun;99:103829. doi: 10.1016/j.bioorg.2020.103829. Epub 2020 Apr 8. Bioorg Chem. 2020. PMID: 32299018
dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase.
Zasedateleva OA, Vasiliskov VA, Surzhikov SA, Kuznetsova VE, Shershov VE, Guseinov TO, Smirnov IP, Yurasov RA, Spitsyn MA, Chudinov AV. Zasedateleva OA, et al. Among authors: kuznetsova ve. Nucleic Acids Res. 2018 Jul 6;46(12):e73. doi: 10.1093/nar/gky247. Nucleic Acids Res. 2018. PMID: 29648660 Free PMC article.
Combining recombinase polymerase amplification with tyrosine modified 2'-deoxyuridine-5'-triphosphate for direct voltammetric detection of double-stranded DNA: Application to potato pathogen Dickeya solani.
Suprun EV, Khmeleva SA, Duskaev IF, Ptitsyn KG, Kurbatov LK, Shershov VE, Kuznetsova VE, Lapa SA, Chudinov AV, Radko SP. Suprun EV, et al. Among authors: kuznetsova ve. Talanta. 2024 Jun 1;273:125841. doi: 10.1016/j.talanta.2024.125841. Epub 2024 Feb 29. Talanta. 2024. PMID: 38460421