Whole-mouse clearing and imaging at the cellular level with vDISCO.
Cai R, Kolabas ZI, Pan C, Mai H, Zhao S, Kaltenecker D, Voigt FF, Molbay M, Ohn TL, Vincke C, Todorov MI, Helmchen F, Van Ginderachter JA, Ertürk A.
Cai R, et al.
Nat Protoc. 2023 Apr;18(4):1197-1242. doi: 10.1038/s41596-022-00788-2. Epub 2023 Jan 25.
Nat Protoc. 2023.
PMID: 36697871
Review.
Tissue clearing methods, together with recent advances in microscopy, have made holistic examinations of biological samples feasible. Here, we report the detailed protocol for nanobody(V(H)H)-boosted 3D imaging of solvent-cleared organs (vDISCO), a pressure-driven, nanobod …
Tissue clearing methods, together with recent advances in microscopy, have made holistic examinations of biological samples feasible. Here, …