Study on the Mechanism of miR-146a in Gingival Mesenchymal Stem Cells

Fang Wang; Zheng Jing; Ting Wei; Hui Jiang; Xiaojun Li

doi:10.1155/2022/1630260

Study on the Mechanism of miR-146a in Gingival Mesenchymal Stem Cells

Evid Based Complement Alternat Med. 2022 Nov 15:2022:1630260. doi: 10.1155/2022/1630260. eCollection 2022.

Authors

Fang Wang^{1

2}, Zheng Jing³, Ting Wei¹, Hui Jiang^{1

2}, Xiaojun Li^{1

2}

Affiliations

¹ Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou 310006, China.
² Department of Periodontology, the Affiliated Stomatology Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China.
³ Central Hospital of Haining, Jiaxing 314408, China.

Abstract

This study aimed to investigate the molecular mechanisms of microRNA-146a (miR-146a) on gingival mesenchymal stem cells (MSCs). Gingival MSCs were isolated from the gingiva tissues of patients with periodontal disease to reveal the function of miR-146a in regulating osteoblast differentiation. miR-146a inhibits osteoblast differentiation by inhibiting phosphorylated cyclic-AMP response binding (CREB) protein translocation into the nucleus and ultimately attenuating runt-related transcription factor 2 (Runx2) expression. Furthermore, silencing miR-146a promotes the proliferation of gingival MSCs. Of note, targeted inhibition of miR-146a also inhibited LPS-induced inflammatory response and promoted the proliferation of gingival MSCs via CREB/Runx2 axis. MiR-146a is a key negative regulator of gingival MSCs proliferation and osteogenic differentiation, and targeting to reduce the miR-146a expression is essential for bone formation signaling. Therefore, we propose that miR-146a is a useful therapeutic target for the development of bone anabolic strategies.

Publication types

Retracted Publication