Single-drop optimization of protein crystallization

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Aug 1;68(Pt 8):994-8. doi: 10.1107/S1744309112024074. Epub 2012 Jul 31.

Abstract

A completely new crystal-growth device has been developed that permits charting a course across the phase diagram to produce crystalline samples optimized for diffraction experiments. The utility of the device is demonstrated for the production of crystals for the traditional X-ray diffraction data-collection experiment, of microcrystals optimal for data-collection experiments at a modern microbeam insertion-device synchrotron beamline and of nanocrystals required for data collection on an X-ray laser beamline.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Crotalid Venoms / analysis*
  • Crotalus
  • Crystallization
  • Crystallography, X-Ray / instrumentation
  • Crystallography, X-Ray / methods*
  • Marantaceae / chemistry
  • Plant Proteins / analysis*
  • Ribosome Inactivating Proteins, Type 2 / analysis*
  • Toxins, Biological / analysis*
  • Viscum album / chemistry

Substances

  • Crotalid Venoms
  • Plant Proteins
  • Ribosome Inactivating Proteins, Type 2
  • Toxins, Biological
  • mistletoe lectin I
  • crotamine