Hydroxyapatite nano-pillars on TI-6Al-4V: Enhancements in cell spreading and proliferation during cell/surface integration

Sarah Akua Osafo; Precious Osayamen Etinosa; John David Obayemi; Ali Azeko Salifu; Tabiri Asumadu; Desmond Klenam; Benjamin Agyei-Tuffour; David Dodoo-Arhin; Abu Yaya; Winston Oluwole Soboyejo

doi:10.1002/jbm.a.37726

Hydroxyapatite nano-pillars on TI-6Al-4V: Enhancements in cell spreading and proliferation during cell/surface integration

J Biomed Mater Res A. 2024 Apr 17. doi: 10.1002/jbm.a.37726. Online ahead of print.

Authors

Sarah Akua Osafo^{1

2

3}, Precious Osayamen Etinosa³, John David Obayemi^{3

4}, Ali Azeko Salifu⁵, Tabiri Asumadu^{3

6

7}, Desmond Klenam^{3

8}, Benjamin Agyei-Tuffour¹, David Dodoo-Arhin¹, Abu Yaya¹, Winston Oluwole Soboyejo^{3

4

7}

Affiliations

¹ Department of Materials Science and Engineering, University of Ghana, Accra, Ghana.
² Department of Biomaterial Science, Dental School, University of Ghana, Korle Bu Campus, Accra, Ghana.
³ Department of Mechanical Engineering, Program in Materials Science and Engineering, Worcester Polytechnic Institute, Worcester, Massachusetts, USA.
⁴ Department of Biomedical Engineering, Worcester Polytechnic Institute, 60 Prescott Street, Gateway Park Life Sciences and Bioengineering Centre, Worcester, Massachusetts, USA.
⁵ Department of Engineering, Boston College, Chestnut Hill, Massachusetts, USA.
⁶ Department of Materials Engineering, Sunyani Technical University, Sunyani, Ghana.
⁷ Department of Mechanical Engineering, College of Engineering, State University of New York (SUNY) Polytechnic Institute, Utica, New York, USA.
⁸ Academic Development Unit and School of Chemical and Metallurgical Engineering, University of the Witwatersrand, Johannesburg, South Africa.

PMID: 38630051
DOI: 10.1002/jbm.a.37726

Abstract

Despite the attractive combinations of cell/surface interactions, biocompatibility, and good mechanical properties of Ti-6Al-4V, there is still a need to enhance the early stages of cell/surface integration that are associated with the implantation of biomedical devices into the human body. This paper presents a novel, easy and reproducible method of nanoscale and nanostructured hydroxyapatite (HA) coatings on Ti-6Al-4V. The resulting nanoscale coatings/nanostructures are characterized using a combination of Raman spectroscopy, scanning electron microscopy equipped with energy dispersive x-ray spectroscopy. The nanostructured/nanoscale coatings are shown to enhance the early stages of cell spreading and integration of bone cells (hFOB cells) on Ti-6Al-4V surfaces. The improvements include the acceleration of extra-cellular matrix, cell spreading and proliferation by nanoscale HA structures on the coated surfaces. The implications of the results are discussed for the development of HA nanostructures for the improved osseointegration of Ti-6Al-4V in orthopedic and dental applications.

Keywords: accelerated extracellular matrix and cell spreading; hydroxyapatite nanostructures; improved cell spreading and proliferation; orthopedic/dental applications; pack cementation.

Abstract

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